SGTA基因及其蛋白的生物信息学分析  

Bioinformatics analysis of SGTA gene and protein

作　　者：顾取良[1] 陈佳园[2] 郐一贺 胡曦文[2] 杨永霞[1] 王丽京[2] 

机构地区：[1]广东药学院基础学院,广东广州510006 [2]广东药学院血管生物学研究所,广东广州510006

出　　处：《生物技术》2015年第2期138-142,共5页Biotechnology

基　　金：国家自然科学基金项目("二棕榈酰磷脂酸抑制肿瘤血管生成作用及其分子机制研究";No.31100852)资助

摘　　要：[目的]对SGTA基因及其蛋白的结构和特征进行生物信息学分析,为研究SGTA与肿瘤形成和发展的相关性提供理论基础。[方法]运用生物信息学数据库和软件对SGTA基因的结构、单核苷酸多态性位点(SNP)、SGTA基因与其他基因的相互作用网络、SGTA蛋白的理化性质、二级结构、蛋白结构域、蛋白翻译后修饰、蛋白质之间相互作用网络进行分析。[结果]人SGTA基因有5种可变剪接产物,编码区存在78个SNP位点,其中错义突变31个,无义突变1个。人SGTA蛋白由313个氨基酸组成,是稳定性不高的亲水蛋白,α-螺旋是其主要二级结构元件,属于TRP超家族,预测有3个磷酸化激酶修饰位点和数个潜在泛素化修饰位点。与SGTA存在相互作用的基因和蛋白多数与维持体内蛋白质稳定的分子伴侣功能相关。[结论]SGTA基因及其蛋白的生物信息学分析为进一步实验研究其在肿瘤形成和发展中的地位及调控机制奠定了基础。[ Objective] To provide genetic characteristics and the encoding protein structure as well as regulation information for further study on the role of SGTA protein in neoplasm development. [ Methods ] Bioinformatics approaches were applied to analyze SGTA gene structure, single nucleotide polymorphisms, gene interaction network, protein secondary structure, post - translational modification and protein interaction network as well as multiple sequence alignment of SGTA protein with its homologous sequences. [ Results ] There are five alternative transcripts in SGTA gene and 78 SNPs were found in coding region, including 1 nonsense and 31 missense mutations. SGTA protein is comprised of 313 amino acid residues and is an unstable hydrophilic protein. It has one highly conserved domain belonging to TRP superfamily, with the c~ -helix elements as the majority of its secondary structure. Several phosphorylation sites as well as multiple ubiquitination sites are present in the SGTA protein. The interaction network between SGTA and other genes shares three common sites with the interaction network between SGTA and other proteins, suggesting it may regulate protein degradation, cellular localisation, protein - protein interactions and signal transduction. [ Conclusion] The genetic characteristics and the encoding protein structure as well as regula- tion networks were predicted by bioinformatics, which provided insightful information for further study on the role of SGTA in neoplasm de- velopment.

关 键 词：SGTA 生物信息学 共分子伴侣 肿瘤 

分 类 号：Q786[生物学—分子生物学]