鹅喉羚线粒体D-loop和Cytb序列遗传多样性分析  

Genetic diversity of Gazella subgutturosa based on mitochondrial DNA D- loop and Cytb sequence

作　　者：吕雪峰 阿布力孜.阿布力米提 宫平 高维明 段新华 邢巍婷 丁新民 郑文新 

机构地区：[1]新疆维吾尔自治区种羊与羊毛羊绒质量安全监督检验中心,新疆乌鲁木齐830000 [2]新疆天山野生动物园,新疆乌鲁木齐830000

出　　处：《生物技术》2015年第2期165-168,184,共5页Biotechnology

基　　金：乌鲁木齐市科学计划项目("通过毛发研究开发鹅喉羚等五种珍稀野生动物健康养殖技术";No.Y111310017)资助

摘　　要：[目的]为了探讨新疆天山野生动物园17只鹅喉羚的遗传多样性、亲缘关系和群体来源。[方法]对这些鹅喉羚线粒体控制区(D-loop)和细胞色素b(Cytb)基因序列进行了PCR扩增和测序,分别计算了群体单倍型多样度(H)和核苷酸多样性(Pi)以及个体间的遗传距离,并构建了单倍型NJ(Neighbor-Joining)聚类图。[结果]D-loop区和Cytb分别获得了978bp和543bp的基因片段,D-loop区识别出11个单倍型,Cytb区识别出3个单倍型;D-loop和Cytb的单倍型多样度(H)和核苷酸多样性(Pi)分别为0.863、0.01028和0.330、0.00077;个体之间最大遗传距离为0.024,最小遗传距离为0.001;17只鹅喉羚分别聚为2个分支。[结论]该鹅喉羚群体D-loop区多态性较丰富,在野外可能来自两个不同的群体。[ Objective] This research was to investigate genetic diversity, relationship and population source of 17 Gazella subgutturosas that selected from Xinjiang Tianshan wild animals park. [ Methods] Sequences of mitochondrial D - loop and Cytb gene in the 17 individu- als were amplified and analyzed. Then haplotype diversity （ H）, nucleotide diversity （Pi） and genetic distance were calculated respective- ly. Additionally, phylogenetic tree was constructed by MEGA 5.0 software with NJ method. [ Results ] 978 bp of D - loop sequence and 543 bp of Cytb sequence were obtained. There were 11 distinct haplotypes D - 1 oop sequences and 3 distinct haplotypes in Cytb se- quences. H and Pi of D - loop and Cytb sequences were 0. 863, 0. 01028 and 0. 330, 0. 00077, respectively. The maximum and minimum genetic distance was 0. 024 and 0. 001, respectively. The 17 Gazella subgutturosas were clustered into 2 branches. [ Conclusion ] This group of Gazella subgutturosas is rich in genetic diversity. It suggests that they may come from 2 different populations.

关 键 词：鹅喉羚 线粒体DNA D-LOOP CYTB 遗传多样性 

分 类 号：Q953[生物学—动物学]