基于数字基因表达谱筛选黄瓜中水杨酸诱导基因  

Screening of Genes Induced by Salicylic Acid in Cucumber Based on Digital Gene Expression Profiling

作　　者：刘双[1] 乔禹[1] 王芳[1] 韩旭[1] 金晓霞[1] 池春玉[1] 于丽杰[1] 丁国华[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院/黑龙江省高校植物学重点实验室,黑龙江哈尔滨150025

出　　处：《核农学报》2015年第5期874-884,共11页Journal of Nuclear Agricultural Sciences

基　　金：黑龙江省高校科技创新团队研究计划资助项目(KJTD-2011-2);植物生物学黑龙江省高校重点实验室(哈尔滨师范大学)开放课题(ZK201201)

摘　　要：本文通过筛选水杨酸诱导黄瓜叶片差异表达基因(DEGs),旨在揭示水杨酸提高黄瓜抗病性机制和信号转导过程。以抗霜霉病黄瓜东农649为试材,无菌培养幼苗至4叶期,喷施5 mmol·L-1的水杨酸,喷施前和喷施后3、12和24 h分别采取叶片,提取总RNA,制备c DNA文库,应用Illumina高通量测序技术对水杨酸处理前后的4个叶片c DNA文库进行差异基因数字表达谱分析。结果表明,SA诱导了多数与叶绿体和细胞壁相关基因的下调表达和多个与抗病相关基因的上调表达,对受体激酶、细胞色素P450、脂氧合酶和脂转运蛋白等信号转导相关基因的表达均有显著影响。本研究获取了丰富有效的数据,初步筛选的DGEs涉及到植物的PCD(程序性细胞死亡)、信号转导、系统获得性抗性(SAR)形成等过程,证实了SA与上述过程有密切关系,为最终揭示SA提高黄瓜抗病性机制奠定了基础。In this paper, the mechanism of disease resistance increased by salicylic acid （SA） and SA signal transduction in cucumber was investigated by screening differentially expressed genes （DEGs） induced by SA. Approximately 5 mmol·L^-1 SA was sprayed on ＂DongNong 649＂used as test material at the four-leaf stage of aseptic seedlings. The leaves were collected before sprayed with SA and after 3, 12, and 24 h respectively. Total RNA was extracted and used for cDNA libraries construction. The differential expression profilings of genes in these cDNA libraries were analyzed via Illumina high-throughput sequencing technology. Results showed that SA induced most of chloroplast- and cell wall-related genes up-regulated expression and multiple genes related to disease resistance down- regulated expression. Also SA significantly affected signal transduction-regulated genes, such as receptor kinases, cytochrome P450, lipoxygenase, and lipid transport protein. This study obtained the effective data, preliminary screening DGEs involved in plant PCD （programmed cell death） , signal transduction and system acquired resistance （SAR） forming, confirmed that SA had close relationship with the above process, finally laid the foundation for revealing mechanism of SA induced cucumber disease resistance.

关 键 词：黄瓜 水杨酸 数字基因表达谱 高通量测序 差异表达基因 系统获得性抗性 

分 类 号：S642.2[农业科学—蔬菜学]