度洛西汀对早期应激模型大鼠行为及海马和前额皮层星形胶质源性蛋白表达的影响  

Effects of duloxetine on depression behavior and S100B protein expression in hippocampus and prefrontal cortex in rats with early stress model

作　　者：杨坤[1] 于雪[1] 王敬兰[2] 胡义秋[3] 

机构地区：[1]天津市安定医院精神卫生研究所,天津300222 [2]天津市南开医院心身科 [3]湖南农业大学科学技术师范学院心理学系

出　　处：《中国慢性病预防与控制》2015年第5期331-334,共4页Chinese Journal of Prevention and Control of Chronic Diseases

基　　金：国家自然科学基金资助项目(30900484);天津市卫生局重点课题(2010KR10)

摘　　要：目的研究度洛西汀对早期应激模型大鼠行为及海马和前额皮层星形胶质源性蛋白(S100B)的影响。方法选取刚出生的54只清洁级雄性SD大鼠为实验动物,采用早期慢性应激制备36只抑郁模型大鼠,将其随机分为抑郁模型组和度洛西汀组,每组18只;对照组18只。度洛西汀组灌胃给予度洛西汀10 ml/kg,连续28 d,抑郁模型和对照组灌胃给予同体积蒸馏水,连续28 d。采用水迷宫试验、糖水偏爱试验检测大鼠的行为改变。采用免疫印迹法检测各组大鼠海马和前额皮层S100B的表达。结果水迷宫试验结果显示,与抑郁模型组比较,度洛西汀组和对照组平均逃避潜伏期均明显缩短,差异有统计学意义(P<0.05);对照组和度洛西汀组的平均逃避潜伏期差异无统计学意义(P>0.05)。空间搜索试验结果显示,与抑郁模型组(秩次为5.33)比较,度洛西汀组(秩次为11.00)和对照组(秩次为12.17)穿台次数明显增加,差异有统计学意义(P<0.05)。与抑郁模型组偏离角[(53.86±4.24)°]比较,度洛西汀组[(25.38±7.93)°]和对照组[(22.17±3.79)°]偏离角显著减小,差异均有统计学意义(P<0.05)。度洛西汀组和对照组的穿台次数和偏离角差异无统计学意义(P>0.05)。抑郁模型组糖水消耗率(46%±12%)显著小于度洛西汀组(68%±4%)和对照组(63%±11%),差异有统计学意义(P<0.05)。度洛西汀组和对照组的海马S100B表达水平(分别为242.24±2.47、247.50±7.86)和前额皮层S100B表达水平(分别为269.13±11.21、253.01±6.73)显著高于抑郁模型组(海马:160.44±8.71,前额皮层:139.56±6.53),差异均有统计学意义(P<0.05)。结论度洛西汀能够改善早期应激抑郁模型大鼠的行为能力和激活海马和前额皮层星形胶质细胞活性。Objective To study the effects of duloxetine on depression behavior and SIOOB protein expression in hippocampus and prefrontal cortex in rats with early stress model. Methods Fifty-four SD rats were randomly divided into early stress model group （ 18 rats）, early stress model with duloxetine group （ 18 rats） and control group （ 18 rats）, 10 ml/kg duloxetine was given to duloxetine group and distilled water was given to model and control groups by intragastric administration for 28 days. The Morris water maze test and the saccharine preference test were used to measure the behavioral change of rats after 28 days intervention behavioral change of rats after 28 days intervention. Western blotting was used to detect SIOOB protein expression in hippocampus and prefrontal cortex. Results Morris water maze test indicated that the average escape latency in control group and duloxetine group was significantly shorter than that in depressive model group （P〈0.05）, but there was no significant difference of average escape latency between control group and duloxetine group （P〉0.05）. The numbers （11.00 and 12.17 rank） of crossing the platform in duloxetine group and control group were significantly more than that （5.33 rank） in depressive model group （P〈0.05）. The heading angles [ （25.38±7.93） o and （22.17±3.79 ） °] in duloxetine group and control group were significantly smaller than that [ （53.86±4.24）°] in depressive model group （P〈0.05）. In the saccharine preference test, the saccharine preference rates （68%±4% and 63%±11%） in duloxetine group and control group were significantly higher than that （46%±12%） in depressive model group （P〈 0.05）. The S100B protein expression levels （242.24±2.47 and 247.5±7.86） in hippocampus and the S100B protein expression levels （269.13± 11.21 and 253.01±6.73） in prefrontal cortex of duloxetine group and control group were significantly higher than those （160.44±8.71 and 139.56±6.53） of depressive

关 键 词：度洛西汀 早期应激 抑郁 海马 星形胶质源性蛋白 

分 类 号：R338.2[医药卫生—人体生理学]