糖尿病大鼠心和肾组织纤维化超微结构的动态观察  

ULTRASTRUCTURAL DYNAMIC OBSERVATION OF MYOCARDIAL AND RENAL FIBROSIS IN TYPE 1 DIABETES MELLITUS RATS

作　　者：郭淑香[1] 孙冬梅 王淑英[1] 李静[3] 李海朋[4] 薛瑞凤[1] 郭倩茹[1] 

机构地区：[1]河北省唐山工人医院分院,063003 [2]华北理工大学研究生部 [3]唐山市妇幼保健院 [4]唐山协和医院

出　　处：《中国煤炭工业医学杂志》2015年第5期794-798,共5页Chinese Journal of Coal Industry Medicine

基　　金：河北省科技支撑项目(编号:10276105D-48)

摘　　要：目的探讨不同时间点1型糖尿病大鼠模型心、肾组织超微结构的病理变化和特点。方法腹腔注射链尿佐菌素(STZ,65mg/kg)建立SD大鼠1型糖尿病模型,注药后7d造模成功后于4、12、24周心肌、肾脏光镜与电镜观察,并与同龄正常大鼠进行比较。结果与正常对照组比较,电镜发现糖尿病组心肌细胞肌丝稀疏,间质胶原增生;肾组织肾小球毛细血管基底膜弥漫性增厚,间质胶原增生。结论组织纤维化是糖尿病心肌病和糖尿病肾病共同的病理基础,随病程延长而病变加重。Objective To investigate the myocardial and renal pathomorphology at different stages of type I diabetes mellitus （DM） rats. Methods The diabetes mellitus rat models was induced by a single intraperitoneal injection of Streptozotocin （STZ,Sigma） at a dose of 65mg· kg- 1 body weight. The myocardialand renal pathomorphology changes were observed with light microscope and transmission electron micro- scope at weeks of 4, 12 and 24 after DM was induced. The age- matched normal rats served as control group. Results Compared with control rats, myofilament rarefaction, interstitial collagen hyperplasia in diabetic rats were observed by electron microscope. Diffuse thickening of glomerular capillary basement membrane and interstitial collagen hyperplasia in diabetic rats were observed by electron microscope. Conclusion Tissue fibrosis is the common pathologic basis of diabetic cardiomyopathy and diabetic nephropathy, and the pathological changes increase with the disease course of diabetes mellitus lengthening.

关 键 词：糖尿病大鼠 糖尿病心肌病 糖尿病肾病 纤维化 超微结构 

分 类 号：R587.2[医药卫生—内分泌]