人工老化处理对高粱种子萌发及生理生化特性的影响  

Effect of Artificial Accelerated Aging on Germination and Physiological and Biochemical Characteristics of Sorghum Seeds

作　　者：韩熠[1] 周宇飞[1] 高铭悦[1] 张壮[1] 黄瑞冬[1] 

机构地区：[1]沈阳农业大学农学院,辽宁沈阳110161

出　　处：《种子》2015年第5期9-12,16,共5页Seed

基　　金：国家现代农业产业技术体系建设专项资金资助项目(编号:CARS-06-02-02)

摘　　要：目的：探讨人工老化处理对高粱种子萌发特性和相关生理生化指标的影响。方法：以高粱吉杂127和吉杂355为试材,人工老化处理（50℃、RH 100%）高粱种子,测定其发芽指标（发芽率、发芽势、发芽指数和活力指数）和各项生理生化指标。结果：随着老化程度的加深,发芽指标均呈现下降趋势,吉杂127上述各指标在老化处理后与对照相比分别降低4.2%-46.9%、18.0%-51.7%、18.0%-51.7%和29.8%-79.3%,吉杂355相应指标经各老化处理后与对照相比分别降低25.3%-83.2%、10.39%-81.8%、9.9%-81.8%和17.26%-90.9%,下降幅度更为明显。老化过程中,种子中POD活性逐渐减低,而MDA含量、电导率和可溶性蛋白质含量均随老化时间的延长逐渐升高。结论：膜损伤是高粱种子老化的主要原因,其中POD活性和MDA含量变化能很好地反映出种子老化程度。Objective：The objective of the current study was to explore the effects of artificial aging on germination,physical and chemical characteristics of sorghum seeds.Method：Two sorghum hybrids,Jiza 127 and Jiza355 were treated in the condition of high temperature（50 ℃） and high humidity（100% relative humidity）.The germination parameters（germination rate,germination potential,germination index and vigor index,physical and chemical parameters were measured.Result：The result showed that the germination parameters were reduced in aged seeds.The above four germination parameters of Jiza 127 decreased by 4.2%-46.9%,18.0%-51.7%,18.0%-51.7% and 29.8%-79.3%,respectively;and those of Jiza 355 were reduced by 25.3%-83.2%,10.39%-81.8%,9.9%-81.8% and 17.26%-90.9%,respectively.The POD activities of Jiza 127 and Jiza 355 decreased respectively by 86% and 81% after 6 days aging.MDA content,electrical conductivity and soluble protein increased along with aging time,with increase by 74%,47%,53%（Jiza 127） and 44%,47%,30%（Jiza 355） after 6 days aging.Conclusion：The main reason of seeds aging is that the cell membrane systems were compromised.The activities of POD and MDA content can well reflect the aging degree of sorghum seeds.

关 键 词：高粱种子 人工老化 萌发 生理生化特性 

分 类 号：S514[农业科学—作物学]