FX-139发酵液及菌丝体提取物对人参愈伤组织生长及防御酶系的影响  

The Effect of the Fermented Liquid and Mycelium Extract of FX-139 on the Growth and Defense Enzymes of Ginseng callus

作　　者：郭双双[1,2] 史册[1,2] 韩梅[1,2] 杨利民[1,2] 

机构地区：[1]吉林农业大学中药材学院 [2]吉林农业大学省部共建生态恢复与生态系统管理国家重点实验室,长春130118

出　　处：《生物技术通报》2015年第5期128-133,共6页Biotechnology Bulletin

基　　金：国家科技支撑计划项目(2011BAI03B01-02);国家自然科学基金项目(31270371);吉林省科技发展计划重点项目(20125068)

摘　　要：以人参栽培土壤中分离纯化的生防菌株放线菌FX-139的发酵液和菌丝体提取物为供体,研究了其对人参愈伤组织生长的影响,及诱导受体防御酶的能力。结果表明:(1)FX-139发酵液和菌丝体提取物浓度为20 mg/L的处理条件对人参愈伤组织生长促进作用最强,比对照增加了68.96%、51.60%,差异显著。(2)发酵液水饱和正丁醇提取物处理下PAL、POD、PPO酶活峰值分别出现在第21天、第14天、第28天,比CK依次提高了1.29倍、2.5倍、2.12倍。菌丝体丙酮提取物处理下PAL、POD、PPO酶活峰值分别出现在第21天、第14天、第14天,比CK依次提高了1.9倍、2.6倍、1.4倍。(3)通过对人参愈伤组织防御酶活性研究表明,发酵液和菌丝体提取物处理下PAL、POD、PPO酶活性均有明显变化。总体来说,放线菌FX-139发酵液和菌丝体提取物可以诱导人参愈伤组织防御反应的表达,发酵液提取物对POD、PPO有良好的诱导作用,菌丝体提取物对PAL、POD和PPO均有较好的诱导效果。Using the fermented liquid and mycelium extract of actinomycetes FX-139 isolated from ginseng rhizosphere as the donor, the effects of it on the growth and ability of inducing defense enzyme activity of ginseng callus were studied. The results showed that： （ 1 ） The treatment of FX-139 fermented liquid and mycelium extract at the concentration of 20 mg/L had the strongest promoting effect on Ginseng callus growth, increasing 68.96% and 51.60% higher than that in the control, i.e., there was significant difference. （ 2 ） There was an active peak of PAL, POD and PPO in 21 d, 14 d and 28 d respectively under the treatment with water saturation n-butyl alcohol extract of fermented liquid, it raised 1.29 times, 2.5 times, and 2.12 times against the control group respectively. There was an active peak of PAL, POD and PPO in 21 d, 14 d and 14 d respectively under the treatment with acetone extract of myeelium, it raised 1.9 times, 2.6 times, and 1.4 times against the control group respectively. （ 3 ） The change of defense enzyme activities in ginseng callus was detected. PAL, POD and PPO enzyme activity were obviously changed with the treatment of fermented liquid and mycelium extract. Overall, Ginseng callus defense responses could be induced by fermented liquid and mycelium extract of FX-139. Fermented liquid extract could effectively induced POD and PPO, and mycelium extract could effectively induced PAL, POD and PPO.

关 键 词：放线菌 人参愈伤组织 PAL POD PPO 

分 类 号：S567.51[农业科学—中草药栽培]