家福捕鸟蛛粗毒对美洲蜚蠊和小鼠半致死剂量的测定  

Determination of Median Lethal Dose of Selenocosmia Jiafu Crude Venom on American Cockroach and Mouse

作　　者：胡朝暾[1,2] 肖震[1] 黄艳[1] 刘中华[2] 

机构地区：[1]怀化学院生命科学系,湖南怀化418008 [2]湖南师范大学生命科学学院,湖南长沙410081

出　　处：《怀化学院学报》2015年第5期41-44,共4页Journal of Huaihua University

基　　金：国家"973"计划项目"我国特有产毒动物多肽毒素的基础与应用基础研究"(2010CB529800);湖南省教育厅青年基金项目"家福捕鸟蛛钠通道毒素的筛选及其结构与功能研究"(13B087)

摘　　要：家福捕鸟蛛是一种生活在中国广西、云南等省山区,中等个体,产毒量较大蜘蛛新种.研究发现它的毒液中含有许多不同活性的成分.为了更好地开发利用该种生物资源,评估其毒性大小,测定了家福捕鸟蛛粗毒对美洲蜚蠊和小鼠的半致死剂量.家福捕鸟蛛粗毒对美洲蜚蠊具有较强的毒性,低剂量的粗毒对美洲蜚蠊具有一定的毒性,高剂量的粗毒能够使美洲蜚蠊在几分钟内致死.家福捕鸟蛛粗毒对美洲蜚蠊的半致死剂量为85.90μg/g.但家福捕鸟蛛粗毒对小鼠的毒性较弱,当注射剂量达到10 mg/kg体重时小鼠未见明显的中毒症状.当注射的粗毒剂量达到46.30 mg/kg时小鼠在24 min内死亡.家福捕鸟蛛粗毒可能主要是一类选择性作用于昆虫的蜘蛛毒素.Selenocosmia jiafu （S .jiafu） is recently identified as a new species of spider in China .These medium bodied venomous spiders are distributed mainly in the hilly areas of southwest of China ,mostly at Yunnan and Guangxi Province .The venom of this spider contains many components with different types of biological activity . In order to explore the utility of this rich source and assess the crude venom’s toxicity , Median lethal dose of S . jiafu crude venom on Cockroach and mouse was determinate . Toxicity experiments revealed that Intra - abdominal injection of the crude venom had severe toxic effects on cockroaches and caused death at higher concentrations within minutes .The LD50 of crude venom on cockroach was 85.90 μg∕g body weight .Its effect on mice was elusive .There was no observable symptom or behavioral change detected after intra - peritoneal injection of the crude venom even at doses up to 10 mg∕kg body weight .The mouse died after being injected the crude venom of the dose of 46.30 mg∕kg body weight within 24 minutes .The data indicated that the S .jiafu crude venom was a kind of spider toxins affecting on insects selectively .

关 键 词：蜘蛛毒液 家福捕鸟蛛 家福捕鸟蛛粗毒 半致死剂量 

分 类 号：Q965.9[生物学—昆虫学]