黄芩苷对人胃癌SGC-7901细胞增殖抑制作用及其机制研究  

Study on Inhibition Effect and Mechanism of Baicalin on Human Gastric Carcinoma SGC-7901 Cells

作　　者：李峰[1] 王根旺[1] 钱彬彬[1] 杨孙虎[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院普外四科,上海201999

出　　处：《中国药物警戒》2015年第5期257-260,共4页Chinese Journal of Pharmacovigilance

摘　　要：目的探讨黄芩苷对人胃癌SGC-7901细胞增殖抑制、诱导凋亡作用及其分子作用机制。方法 MTT法检测黄芩苷对SGC-7901细胞的增殖抑制作用;PI单染法检测细胞周期;Annexin V/PI双染法检测细胞凋亡;Western blot法检测凋亡相关基因Bcl-2和Bax的蛋白表达水平。结果黄芩苷对SGC-7901细胞生长有明显抑制作用,作用24 h和48 h对SGC-7901细胞的半数抑制浓度(IC50)分别为218.73μmol·L-1,161.86μmol·L-1,呈现时间—剂量依赖性;FCM结果显示黄芩苷能使SGC-7901细胞阻滞在G2/M期;Annexin V/PI双染法验证黄芩苷可以诱导细胞凋亡;Western blot结果显示Bax蛋白水平明显上调,而Bcl-2的蛋白表达水平降低。结论黄芩苷对SGC-7901细胞增殖具有显著的抑制作用并可诱导细胞凋亡,其作用机制与其上调Bax蛋白表达水平的同时,下调Bcl-2蛋白表达水平,从而诱导细胞凋亡有关。Objective To investigate the inhibiting effect and its mechanism of baicalin on human gastric carcinoma SGC-7901 cells. Methods Growth inhibition rates of SGC-7901 cells were measured with MTT method, the cell cycle distribution was detected by PI single staining, apoptosis was detected by Annexin V/PI double staining, and the expression levels of Bcl-2, bax protein were analyzed by western blot. Results Baicalin inhibited the proliferation of SGC-7901 cells in a dose and time dependent manner. The IC50 of 24 h and 48 h were 218.73 μmol·L^-1 and 161.86 μmol·L^-1 respectively. The cell growth was arrested in G2/M phase. Fluorescent staining can detect the apoptotic cells induced by baicalin. The expression of Bax protein was increased while the Bcl-2 was decreased. Conclusion Baicalin not only inhibits proliferation but also induces apoptosis of SGC-7901 cells. Its mechanism is related to down-regulating Bcl-2 while up-regulating Bax.

关 键 词：黄芩苷 胃癌 SGC-7901细胞 增殖抑制 机制 

分 类 号：R965[医药卫生—药理学] R285.5[医药卫生—药学]