广西1例人田鼠巴贝虫感染巢式PCR鉴定及其同事感染调查  

Nest-PCR identification of one human infected of Babesia microti in Guangxi and investigation on his colleagues

作　　者：乔岩[1,2] 彭恒[2] 朱淮民[2] 严继舟[1] 

机构地区：[1]上海海洋大学海洋生物和神经科学研究所,上海201306 [2]第二军医大学病原生物学教研室,上海200032

出　　处：《国际医学寄生虫病杂志》2015年第3期152-155,共4页International JOurnal of Medical Parasitic Diseases

摘　　要：目的 确诊1例国内感染田鼠巴贝虫的病例，对患者周边同事进行调查，初步了解田鼠巴贝虫在广西人群中的分布情况。 方法 提取患者及相关人士血液DNA，共计121份，进行巢式PCR鉴定。以扩增患者18S rRNA基因为分子标记，与GenBank中各巴贝虫序列进行同源性分析，采用最大似然率法（maximum likelihood）构建系统进化树，分析亲缘关系。 结果 患者感染田鼠巴贝虫病，患者周边同事共40人感染，阳性率为33.06%（40/121）；田鼠巴贝虫的系统分类属于感染人的巴贝虫类，与感染牛、犬的巴贝虫亲缘关系较远。 结论 田鼠巴贝虫在患者周边同事中感染率较高，对接触人员具潜在的感染风险，应加强预防和控制。Objective To identify one domestic human case infected with Babesia microti and investi- gate surrounding colleagues so as to understand B. microti distribution among local people in Guangxi Province. Methods A total of 121 blood DNA samples of the patient and his surrounding colleagues was extracted and identified by nested-PCR methods. Then, using patient＇s amplified 18S rRNA as molecular markers, the homology with all Babesia sequences in GenBank was analyzed, and using maximum likelihood method the phylogenetic tree was constructed for analysis of genetic relationship. Results The patient in- fected B. microti disease and so were 40 surrounding colleagues, the positive rate was 33.06%（40/121 ）. The systemic classification of B. microti belongs to the strain of human infection groups and it is distant related to the species of cattle, or dog infection Babesia groups. Conclusion B. microti is of high infection rate among patient＇s surrounding people and it has potential infection risk for contact persons, prevention and control strategy should be strengthened.

关 键 词：田鼠巴贝虫 巢式PCR 分子生物学 

分 类 号：R531.3[医药卫生—内科学]