高效液相色谱法测定感冒热毒清合剂中连翘苷的含量  

Content determination of phillyrin in Ganmao-reduqing Mixture by high performance liquid chromatography

作　　者：邹晓华[1] 何伟康[1] 戴安印[1] 邹杰[1] 张扬[2] 

机构地区：[1]解放军第一一七医院药剂科,浙江杭州310013 [2]浙江省中医药研究院,浙江杭州310007

出　　处：《中国医药导报》2015年第15期117-119,123,共4页China Medical Herald

基　　金：解放军总后勤部制剂科研提高项目(14TG0173)

摘　　要：目的：建立感冒热毒清合剂中连翘苷的高效液相色谱（HPLC）法含量测定方法。方法对处方中连翘进行定性鉴别，并对连翘苷进行HPLC法测定，采用色谱柱资生堂SHISEIDO CAPCELL PAK MG-C18柱（4.6 mm×250 mm，5μm），流动相乙腈-水（76：24），流速1.0 mL/min，柱温30℃，检测波长277 nm。结果连翘苷在6.88~137.6μg/mL范围内呈良好的线性关系（r=0.9992），连翘苷的平均回收率为99.20%、99.96%、98.73%。结论本文首次建立了感冒热毒清合剂中连翘苷的HPLC含量测定方法，结果准确，重复性好，可作为感冒热毒清合剂的质量评价方法。Objective To establish a method on content determination of phillyrin in Ganmao-reduqing Mixture by high performance liquid chromatography （HPLC）. Methods Phillyrin was performed qualitative identification. The content of phillyrin in mixture was determined by HPLC with a SHISEIDO CAPCELL PAK MG-C18 column （4.6 mm×250 mm, 5μm）, acetonitrile-water （76：24） was used as mobile phase and the flow rate was 1.0 mL/min. The columm temperature was set at 30℃ and the wavelength of the detector was set at 277 nm. Results The linear range for phillyrin was 6.88-137.6μg/mL and the correlation cofficient was 0.9992. The average recovery of phillyrin was 99.20%, 99.96%, 98.73%respectively. Conclusion This is the first time to establish a method using HPLC for determination of phillyrin in Gan-mao-reduqing Mixture. The method is accurate, reproducible and suitable to be used for quality assessment of Gan-mao-reduqing Mixture.

关 键 词：感冒热毒清合剂 连翘苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]