高效液相色谱-蒸发光散射检测器法测定中药茺蔚子中盐酸水苏碱的含量  

Content determination of the hydrochloric acid stachydrine in Mother-wort Fruit by HPLC-ELSD

作　　者：张彦飞[1] 李智萌[1] 蔡广知[1] 刘凤臣[1] 贡济宇[1] 高文义[1] 

机构地区：[1]长春中医药大学,吉林长春130117

出　　处：《中国医药导报》2015年第15期124-127,共4页China Medical Herald

基　　金：吉林省教育厅"十二五"科学技术研究项目(2013108)

摘　　要：目的：建立中药茺蔚子中盐酸水苏碱的高效液相色谱-蒸发光散射检测器测定方法。方法 Asahipak NH2P 504E色谱柱（4.6 mm×250 mm），流动相为乙腈-0.2%磷酸溶液（84：16），体积流量1 mL/min，柱温30℃。结果盐酸水苏碱在0.487~4.383μg范围内线性关系良好，r=0.9999，平均回收率为97.96%，RSD为0.92%。结论该法灵敏度高，专属性强，可作为中药茺蔚子中盐酸水苏碱的定量测定方法。Objective To establish HPLC-ELSD determination method of the hydrochloric acid stachydrine in Mother-wort Fruit. Methods The separation by HPLC-ELSD was performed on Asahipak NH2P 50 4E column （4.6 mm×250 mm） with acetonitrile-0.2% phosphoric acid solution （84：16） as mobile phase, 1 mL/min of flow rate and 30℃ of column temperature. Results Hydrochloric acid stachydrine at 0.487-4.383μg had good linear relationship, r=0.9999, the av-erage recovery was 97.96%, RSD was 0.92%. Conclusion The method is sensitive, specific, it can be used as quantita-tive determination method of the hydrochloric acid stachydrine in Motherwort Fruit.

关 键 词：茺蔚子 盐酸水苏碱 高效液相色谱-蒸发光散射检测器法 

分 类 号：R284.1[医药卫生—中药学]