Isolation and Identification of the Pathogens of Bullfrog Streptoccus Disease  

作　　者：Hang Xiaoying Zhou Dongren Luo Yizhi Shi Weida Ye Xueping 

机构地区：[1]Zhejiang Institute of Freshwater Fisheries [2]Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture [3]Key Laboratory of Fish Health and Nutrition of Zhejiang Province

出　　处：《Animal Husbandry and Feed Science》2015年第1期36-38,共3页动物与饲料科学（英文版）

基　　金：Supported by Scientific and Technological Project of Huzhou City(2009GN03);Major Agricultural Program in Zhejiang Province(2009C12056)

摘　　要：The tested strain from typical clinical cases,and the physio-chemical characteristics,16 S rRNA gene sequence and drug sensitivity challenge property were analyzed.This isolate was determined as Streptococcus agalactiae,16 S rRNA gene fragment of 1 280 bp in length.Blast analysis showed that this fragment shared a 100% homology with the conservative region of Streptococcus agalactiae isolate（AB023574）,Streptococcus dysqalactiae isolate（HM146084.1,AB537922,EU075033）,Streptococcus difficilis isolate（AB112407） accessed in Genbank.The isolate was sensitive to ciprofloxacin,neomycin,spectinomycin,furadantin and enoxacin.The results lay foundation for the effective control,rational medication and vaccine preparation for Streptococcus disease on bullfrogs.The tested strain from typical clinical cases,and the physio-chemical characteristics,16 S rRNA gene sequence and drug sensitivity challenge property were analyzed.This isolate was determined as Streptococcus agalactiae,16 S rRNA gene fragment of 1 280 bp in length.Blast analysis showed that this fragment shared a 100% homology with the conservative region of Streptococcus agalactiae isolate（AB023574）,Streptococcus dysqalactiae isolate（HM146084.1,AB537922,EU075033）,Streptococcus difficilis isolate（AB112407） accessed in Genbank.The isolate was sensitive to ciprofloxacin,neomycin,spectinomycin,furadantin and enoxacin.The results lay foundation for the effective control,rational medication and vaccine preparation for Streptococcus disease on bullfrogs.

关 键 词：Bullfrog Streptococcus agalactiae Pathogen 16S rRNA 

分 类 号：S947.2[农业科学—水产养殖]