川续断野生种质资源遗传多样性的SCoT分析  

Analysis on genetic diversity among wild Dipsacus asperoides by SCoT

作　　者：陈大霞[1,2,3,4,5] 张雪[1,2,3,4] 崔广林[1,2,3,4] 王钰[1,2,3,4] 李隆云[1,2,3,4] 张泽[5] 

机构地区：[1]重庆市中药研究院,重庆400065 [2]重庆市中药良种选育与评价工程技术研究中心,重庆400065 [3]重庆市中药资源学重点实验室,重庆400065 [4]中国中医科学院中药资源中心重庆分中心,重庆400065 [5]重庆大学生命科学学院,重庆400044

出　　处：《中国中药杂志》2015年第10期1898-1903,共6页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目(2006BAI06A11-09);国家科技部科技人员服务企业行动项目(2009GJF10045);重庆市"科技平台与基地建设"项目(cstc2014ptyjd10001)

摘　　要：采用目标起始密码子多态性（SCo T）技术对66份川续断种质进行多态性检测,揭示野生川续断种质资源的遗传多样性。用TREECONW软件计算遗传距离,UPGMA方法构建亲缘关系树状图。结果表明,20条引物共检测到181个位点,其中109个为多态位点,占60.13%;遗传距离变化范围在0.030 6~0.181 4;聚类结果显示野生川续断种质分类与地理来源无明显相关性。野生川续断的遗传多样性水平较高,为改良种质资源提供了丰富的遗传基础。To investigate the genetic diversity among wild Dipsacus asperoides in China, 66 germplasmic resources of D. asperoides were analyzed by Start Codon Targeted Polymorphism （SCOT） molecular markers. Genetic distance was calculated by TREECONW software and the systematic diagram of genetic relationship was clustered by UPGMA method. The results showed that the totals of 181 bands were detected using 20 primers , among which 109 were polymorphic bands. The average percentage of polymorphic bands was 60.13%. Genetic distance changed from 0. 030 6 to 0. 181 4. The clustering results showed that there was no significant correlation between the classification of the wild D. asperoides and their geographical origin. The relatively high genetic diversity of D. asperoides provides the basis for breeding new varieties.

关 键 词：川续断 种质资源 遗传多样性 SCO T 

分 类 号：S567.239[农业科学—中草药栽培]