10种微生物对含马兜铃酸A中药材的生物减毒研究  

Research on biological detoxification of Chinese medicine containing aristolochic acid A by ten microorganisms

作　　者：曹艺[1,2,3] 谭周进[1] 夏伯候[1,2,3] 谢嘉驰 林丽美[1,2,3] 廖端芳[1,2,3] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省中药不良成分快速检测及脱除工程技术研究中心,湖南长沙410208 [3]中药有毒物质防控技术湖南省工程实验室,湖南长沙410208

出　　处：《中国中药杂志》2015年第10期1939-1944,共6页China Journal of Chinese Materia Medica

基　　金：湖南省发改委项目(湘发改高技[2013]1199号);湖南省湖湘青年人才项目(湘财教指[2013]158号);湖南省科技厅项目(湘科计字[2012]93号);长沙市科技计划重点项目(k1406030-31);湖南省十二五重点学科药学学科项目

摘　　要：利用微生物对含马兜铃酸A中药材进行生物转化脱毒,筛选出合适的菌种,为后期进一步研究微生物法对含马兜铃酸类中药材的"去毒存效"奠定基础。采用UPLC对12种含马兜铃酸类中药材进行检测,其中有4种中药材检测到含有马兜铃酸A,分别是关木通、青木香、马兜铃和寻骨风,另外8种则未检测到。选取了黑曲霉、米曲霉等10个菌种,以关木通、青木香、马兜铃和寻骨风药材粉末为原料,运用液体发酵技术,在一定条件下进行生物转化脱毒,观察菌种的生长情况,采用UPLC测定发酵前后药材中马兜铃酸A的含量。结果表明有1个菌种对降低关木通、青木香、马兜铃、寻骨风中的马兜铃酸A含量均有一定的效果。This paper was aim to screen microorganisms with attenualed efficiency for Chinese medicine containing aristolochic acid A by liquid-state fermentation. Twelve Chinese medicine were detected by UPLC and aristolochic acid A was only founded in four species of Aristolochia,those were Caulis Aristolochiae Manshuriensis, Aristolochiae Radix,Aistolochia Contorta Bunge and Herba Aristolochiae Mollissima, but not in the others. With the four Chinese medicine containing aristolochic acid A as raw material, ten microorganisms were tested, and the content of aristolochic acid A was detected by UPLC. The results showed that one microorganism can decrease content of aristolochic acid A in all those four Chinese medicine.

关 键 词：马兜铃酸A 生物转化 UPLC 关木通 青木香 马兜铃 寻骨风 

分 类 号：R283[医药卫生—中药学]