参莲提取物对PM_(2.5)染毒RAW264.7细胞损伤的保护作用  

Protection of Shenlian extracts to PM_(2.5) infected RAW 264.7 cell damage

作　　者：刘栩岑 李玉洁[2] 王娅杰[2] 李琦[2] 杨庆[2] 翁小刚[2] 陈颖[2] 蔡维艳[2] 郭琰[2] 阚晓溪[2] 陈熹[2] 黄鹤飞[2] 朱晓新[1,2] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2015年第10期1977-1983,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(30930114;30973901);科技部第二十次中泰科技合作联委会长期合作项目(20-602J)

摘　　要：观察中药参莲(SL)提取物对PM2.5染毒RAW264.7细胞损伤的保护作用。建立PM2.5染毒致RAW264.7细胞损伤模型。以细胞生长、细胞损伤标记物、氧化应激相关因子及炎性细胞因子为观察指标,评价SL提取物对PM2.5染毒RAW264.7细胞损伤的保护作用。结果表明,PM2.550 mg·L-1作用4,24 h时可致细胞颗粒沉积,抑制细胞生长,可显著增加细胞上清中LDH,NO释放量及细胞内活性氧(ROS)水平。在SL提取物50,25,10 mg·L-1干预作用下,RAW264.7细胞颗粒沉积减轻,细胞上清中LDH,NO,IL-1β,MCP-1释放量显著下降,SOD活性显著有所增加。表明SL提取物对PM2.5染毒RAW264.7细胞损伤有明显保护作用,其作用可能与对细胞的直接保护作用、减少氧化应激及炎症损伤有关。The aim of this research is to investigate the protection of PM2. 5 infected RAW264.7 cell by traditional Chinese medicine （TCM） Shenlian（SL） extracts and to establish the damage model. We use cell growth, cell damage and oxidative stress related markers, and inflammatory eytokines as observation index to evaluate the protection of PM2.5 infected RAW264. 7 by SL extract. The results showed that 50 mg · L^- 1 pM2.5 could cause cell particle deposition, inhibit the growth of cells, and significantly increase the cell supernatant of LDH, NO release quantity and intraeellular reactive oxygen species （ROS） level during 4 h and 24 h. In the intervention of SL extract 50,25,10 mg · L^-1, the particle deposition of RAW264.7 cells, cell supernatant of LDH, NO, IL-1 beta release, MCP-1 was significantly decreased, the SOD activity increased significantly. It shows that SL extracts of PMz5 infected RAW264.7 cell damage has obvious protective effect, the effect may be related to the direct protection of cells, reduce oxidative stress and inflammatory injury.

关 键 词：PM2.5 RAW264.7 参莲提取物 

分 类 号：R285[医药卫生—中药学]