塞络通胶囊对局灶型脑缺血/再灌注大鼠线粒体动力学的影响  

Effect of Sailuotong capsule on mitochondrial dynamics in focal cerebral ischemia / reperfusion rats

作　　者：张业昊 丛伟红[1] 徐立[1] 杨斌[1] 姚明江[1] 宋文婷[1] 刘建勋[1] 

机构地区：[1]中国中医科学院西苑医院基础医学研究所,中药药理北京市重点实验室,北京100091

出　　处：《中国中药杂志》2015年第10期1984-1988,共5页China Journal of Chinese Materia Medica

基　　金：国家“重大新药创制”科技重大专项(2012ZX09101214);北京市十病十药(Z121102001112006)

摘　　要：观察塞络通胶囊对局灶性脑缺血再灌注的保护作用机制。采用大鼠90 min中脑动脉闭塞(middle cerebral artery occlusion,MCAO)再灌注模型。蛋白免疫印迹法检测线粒体分裂融合基因Drp1,Opa1表达。透射电镜观察线粒体超微结构变化。HE染色观察病理学形态变化,免疫组织化学法检测Drp1,Opa1表达。结果显示,塞络通胶囊(33,16.5 mg·kg-1,ig)可抑制缺血侧皮层区线粒体分裂融合异常,抑制线粒体分裂基因Drp1表达,促进线粒体融合基因Opa1表达,最终减轻缺血再灌注带来的能量代谢紊乱。塞络通胶囊可抑制缺血周边区线粒体动力学异常、维持线粒体正常形态,说明维持线粒体动力学正常可能是中药塞络通胶囊促进缺血脑区自修复功能的作用机制。To observe the protective effect and mechanism of Sailuotong capsule in focal cerebral ischemia/reperfusion. The 90 min middle cerebral artery occlusion （MCAO） reperfusion model was established. The expressions of dynamin-related protein 1 （ Drpl ） and optic atrophy 1 （ Opal ） were tested by Western blot. The transmission electron microscope was used to observe the changes in the mitochondrial ultra-structure. The pathological morphological changes were observed through the HE staining. The immunohistochemical method was used to test Drpl and Opal expressions. Sailuotong capsule （33, 16. 5 mg · kg^-1 , ig） can inhibit the abnormal mitochondrial fission and fusion in the cortical area on the ischemia side and the mitochondrial fission gene expression and promote the mitochondrial fusion gene Opal expression, so as to alleviate the energy metabolism disorder caused by ischemia/reperfusion. Sailuotong capsule can inhibit the abnormal mitochondrial dynamics in peri-ischemic regions and maintain the normal morphology of mitochondria, which may be the mechanism of Sailuotong capsule in promoting the self-recovery function in the ischemic brain region.

关 键 词：塞络通胶囊 缺血/再灌注 能量代谢 线粒体分裂/融合 

分 类 号：R285.5[医药卫生—中药学]