缺血后处理对大鼠肾缺血再灌注损伤后CD105及endostatin表达的影响  

Effect of ischemic postconditioning on the expression of CD105and endostatin after renal ischemia-reperfusion injury in rats

作　　者：李爱军[1] 喻俊峰[1] 孙德明[1] 蒋林涛[1] 张满[1] 易成[1] 汪志顺[2] 

机构地区：[1]三峡大学人民医院(宜昌市第一人民医院)泌尿外科,湖北宜昌443000 [2]武汉大学人民医院泌尿外科

出　　处：《临床泌尿外科杂志》2015年第5期452-455,共4页Journal of Clinical Urology

摘　　要：目的:探讨缺血后处理对大鼠缺血再灌注损伤后CD105及endostatin表达的影响。方法:将雄性Wistar大鼠随机分为三组,分别为假手术组(Sham组)、缺血再灌注损伤组(IRI组)及缺血后处理组(IPO组)。采用全自动生化分析仪检测血清尿素氮(BUN)及肌酐(Cr)含量;用HE染色观察病理组织学变化;用免疫组织化学法和蛋白质印迹法(Western blot)检测肾组织中CD105及endostatin的表达。结果:IRI组血清BUN及Cr含量较Sham组明显升高,IPO组血清BUN及Cr含量介于Sham组和IRI组之间;HE染色检测发现IRI组肾小管上皮细胞坏死明显;肾小管管腔扩张,IPO组较IRI组肾小管上皮坏死明显减轻;免疫组织化学以及Western blot检测均发现CD105在Sham组表达最强,IRI组表达明显减弱,IPO组表达则介于前二者之间;endostatin在Sham组表达最弱,在IRI组最强,IPO组则稍强于Sham组而明显弱于IRI组。结论:缺血后处理能减弱缺血再灌注损伤过程中endostatin的表达量,同时增加CD105的表达,因而有助于减轻肾脏的缺血再灌注损伤。Objective：To explore the effect of ischemic postconditioning on CD105 and endostatin after renal is- chemia-reperfusion injury in Wistar rats. Method：The male Wistar rats were randomly divided into three groups.. Sham group, ischemia-reperfusion injury （1RI） group, ischemic postconditioning （IPO） group. Serum blood urea nitrogen （BUN） and creatinine （Cr） were analyzed by automatic biochemical analyzer. Histopathological changes were observed by HE staining. The expression of CD105 and endostatin in kidney were checked by immunohisto- chemistry and Western blot. Result：Serum BUN and serum Cr in IRI group were significantly higher than those in Sham group, while serum BUN and serum Cr of IPO group were between the Sham group and IRI group. Signifi- cant necrosis of tubular epithelial cells and tubular lumen expansion could be found in hematoxylin in IRI group. Compared with IRI group, tubular necrosis significantly reduced in IPO group. The result of immunohistochemis- try showed that the expression of CD105 was the strongest in Sham group, and significantly decreased in the IRI group, and was between them in IPO group, while the expression of endostadn was the weakest in the Sham group, the strongest in IRI group, and IPO group＇s expression was slightly stronger than the Sham group＇s but was significantly weaker than IRI group＇s. Western blot test further supported the results of immunohistochemis- try. Conclusion：lschemic postconditioning could reduce the expression of endostatin after IRI, and enhance the ex- pression of CD105, which helps to reduce kidney iniury.

关 键 词：缺血后处理 缺血再灌注损伤 CD105 ENDOSTATIN 大鼠 

分 类 号：R692[医药卫生—泌尿科学]