类人H3N2犬流感病毒广西分离株的鉴定及其遗传演化分析  

Identification and genetic evolution analysis of human-like H3N2 canine influenza virus in Guangxi

作　　者：陈樱[1] 莫彦宁 周华波 肖雄[1] 於庆雄 杨文娟[1] 韦祖樟[1] 谢婷 韦志文 侯韶毅 黄伟坚[1] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530004 [2]广西玉林市动物疫病预防控制中心,广西玉林537000 [3]柳州市鱼峰区华波宠物诊疗中心,广西柳州540005 [4]河池市动物疫病预防控制中心,广西河池547000

出　　处：《中国预防兽医学报》2015年第5期401-404,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家现代化农业产业技术体系广西创新团队生猪产业(nycytxgxcxtd-03-15-1);广西教育厅高校科学技术研究项目(YB2014005);广西大学科研基金项目(XJZ140236)

摘　　要：为了解广西各地区宠物犬流感病毒（CIV）的流行情况,本研究从9个地级市宠物医院采集了326份犬鼻拭子样品,经RT-PCR检测为CIV阳性后对其进行MDCK细胞分离病毒,并对分离株进行全基因组测序和序列分析。结果表明获得2株CIVs分离株,均属于人源谱系,与Moscow/10/99（H3N2）人流感病毒株的同源性大于99%。8个基因节段的核苷酸序列遗传演化分析显示,这2株CIVs与人源H3N2亚型猪流感病毒（SIV）最为相近,同在Moscow/99分支。HA1蛋白氨基酸位点分析显示aa190~aa196位点处仍保留SIV的受体相关结合位点（DQISVYA）,但却改变了流行于人类的部分受体结合位点（DQTSLYT）。本研究为有效预防人-犬流感病毒传播提供了实验依据。To inverstigate the prevalence of canine influenza virus （C/V） among pet dogs in Guangxi, a total of 326 nasal swabs of pet dogs from 9 cities were collected and subject to detection by RT-PCR. The positive samples were inoculated in MDCK ceils for virus isolation, and then two CIVs isolated and sequenced for analysis. The alignment results indicated that 2 CIVs belonged to human-like lineage, with higher homology （〉99%） compared with Moscow/10/99 （H3N2）. Phylogenetic analysis of all eight sagments of the two isolates revealed that they had closer relationship with H3N2 swine influenza viruses, which fell into the branch of Moscow/99. The receptor binding sites （aa190-aa196） of HA1 in the CIVs were conserved with H3N2 swine influenza viruses （DQISVYA）, but partially changed in human binding sites （DQTSLYT）.

关 键 词：宠物犬 类人H3N2亚型 流感病毒 遗传演化 

分 类 号：S852.65[农业科学—基础兽医学]