孕激素对人绒毛滋养层细胞表达ADAM10、Ob-R及分泌SLR、LEP的影响  

Progesterone on the expression of ADAM10,Ob-R and the secretion of SLR,LEP in human chorionic trophoblast cells

作　　者：甘蕾[1] 李真[1] 吴晓华[1] 应德美 陈莉萍[1] 

机构地区：[1]第三军医大学新桥医院妇产科,重庆400037

出　　处：《局解手术学杂志》2015年第3期249-252,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金(30973203)

摘　　要：目的探讨不同浓度孕激素对早孕人绒毛滋养层细胞表达基质金属蛋白酶10(ADAM10)、长型瘦素受体(Ob-R)及分泌可溶性的瘦素受体(SLR)、瘦素(LEP)的影响。方法培养原代人绒毛滋养层细胞,分别给予含有不同浓度的孕激素(0、100、150、200 ng/m L)培养24 h,检测细胞内ADAM10和Ob-R的相对表达量及上清液中SLR及LEP的含量。结果随着孕激素浓度的增加,早孕人绒毛滋养层细胞表达ADAM10的含量逐渐降低,组间两两比较有统计学差异(P<0.05)。随着孕激素浓度的增加,早孕人绒毛滋养层细胞表达Ob-R的含量逐渐增加,组间比较无统计学差异(P>0.05)。各组细胞上清液中SLR含量随着孕激素的浓度增高而逐渐降低,组间两两比较有统计学差异(P<0.05)。各组细胞上清液中LEP浓度随着孕激素浓度的增高而逐渐增高,组间两两比较有统计学差异(P<0.05)。采用Pearson检验发现SLR的表达与LEP的表达存在显著性负相关(R=-0.949,P<0.01)。结论孕激素可能通过影响ADAM10、SLR、LEP的表达而调控瘦素功能的发挥,参与妊娠期糖尿病(GDM)发生。Objective To investigate the different concentrations of progesterone on the expression of a disintegrin and metalloprotease 10 （ADAM10）,long form leptin receptor （Ob-R） and the secretion of soluble leptin receptor （SLR）,leptin （LEP） in primary pregnancy human chorionic trophoblast cells. Methods Cultured primary human trophoblast cells and added in different concentrations of progesterone （0,100,150 and 200 ng/mL） for 24 hours. The relative expression of ADAM10 and Ob-R in the cells and the content of SLR and LEP in the supernatant were detected. Results With increasing concentrations of progesterone,early human trophoblast cells ADAM10 content gradual-ly decreased,the difference between the two groups was significant （P0. 05）. SLR content of the cell supernatants as the concentration of progesterone increased and decreased,there are significant differences between each two groups （P〉0. 05）. LEP cell supernatant in each group with the increase of the concentration of progesterone concentration increased gradually between the two groups were significantly different（P〉0. 05）. Pearson’s test showed that the expression of expression SLR and LEP exists a significant negative correlation （R= -0. 949,P〈0. 01）. Conclusion Progesterone may influence the ex-pression of ADAM10,SLR and LEP by the regulation of leptin to participate GDM occurs.

关 键 词：孕激素 基质金属蛋白酶10 长型瘦素受体 可溶性的瘦素受体 瘦素 

分 类 号：R714.256[医药卫生—妇产科学]