罗格列酮对高糖环境下人脐静脉内皮细胞作用的研究  

Effects of rosiglitazone on activation of human umbilical vein endothelial cells in high glucose environment

作　　者：杨明璨[1] 熊鸿燕[2] 林辉[3] 

机构地区：[1]第三军医大学基础部人体解剖学教研室,重庆400038 [2]第三军医大学军事预防医学院流行病学教研室,重庆400038 [3]第三军医大学军事预防医学院热带研究所,重庆400038

出　　处：《局解手术学杂志》2015年第3期253-256,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金面上项目(31470046)

摘　　要：目的研究罗格列酮对高糖环境下人脐静脉内皮细胞促血管生成功能的影响及其机制。方法通过MTT检测法、细胞划痕实验,探讨罗格列酮在高糖环境下对人脐静脉内皮细胞(HUVEC)的增殖与迁移能力的影响,并用ELISA方法检测经罗格列酮作用后高糖培养基上清中VEGF、SDF-1的含量,进行统计分析。结果经罗格列酮药物处理后的HUVEC增殖和迁移能力明显高于高糖组,且培养基中VEGF和SDF-1的含量也有显著升高。AKT阻断剂可以阻断罗格列酮对HUVEC增殖、迁移和分泌功能的促进作用。结论罗格列酮在高糖环境下可显著促进HUVEC的增殖、迁移和分泌功能,AKT信号通路在这一过程中发挥着重要的作用。Objective To investigate the effects of rosiglitazone on proangiogenesis function of human umbilical vein endothelial cells （ HUVEC） in high glucose environment. Methods HUVECs were cultured in high glucose environment and stimulated by rosiglitazone. MTT, cell scratch test and Transwell assay were used to detect HUVEC proliferation and migration. The concentration of VEGF, SDF-1 was also detected in the supernatant. Results Rosiglitazone could effectively promote HUVEC proliferation and migration. The concentration of VEGF and SDF-1 in rosiglitazone stimulated supernatant was higher than that in high glucose group. The inhibition of AKT signal could block the promotion of rosiglitazone on the HUVEC proliferation, migration and secretion. Conclusion Rosiglitazone could significantly promote HUVEC secretion, proliferation and migration in high glucose environment. AKT signal played an important role in this process.

关 键 词：罗格列酮 人脐静脉内皮细胞 高糖环境 活化 

分 类 号：R459.5[医药卫生—治疗学]