麦冬皂苷B对A549细胞株体外黏附、侵袭及迁移的抑制作用及机制研究  

Inhibitory effect of ophiopogonin-B on adhesion,invasion and migration of A549 cells in vitro

作　　者：陈美娟[1] 赵若琳[1] 郭园园[1] 宁利敏[1] 张旭[1] 

机构地区：[1]南京中医药大学江苏省中医药防治肿瘤协同创新中心,江苏南京210023

出　　处：《中国药理学通报》2015年第5期660-664,共5页Chinese Pharmacological Bulletin

基　　金：江苏省自然科学基金资助项目(No BK20131415);江苏省高校优势学科建设工程项目(PAPD);欧盟第七框架项目(No PIRSES-GA-2013-612589)

摘　　要：目的探究麦冬皂苷B(ophiopogonin-B,OP-B)抑制非小细胞肺癌细胞株A549黏附、侵袭、迁移的作用及其机制。方法细胞黏附实验、Transwell小室实验分别检测细胞黏附、迁移和侵袭能力。通过荧光实时定量反转录PCR(qRT-PCR)检测OP-B对A549细胞中MMP-2/9 mRNA表达水平的调控,Western blot法检测细胞中MMP-2/9蛋白及其上游调控蛋白Akt及其磷酸化水平的变化。结果 10μmol·L-1浓度的OP-B能明显抑制A549细胞黏附、侵袭和迁移,同时能抑制其MMP-2/9 mRNA及蛋白水平的表达,并抑制其上游p-Akt的表达。结论 OP-B有效抑制A549细胞黏附、侵袭和迁移,其作用与下调MMP-2/9 mRNA和蛋白水平的表达及抑制其上游Akt的磷酸化有关。Aim To explore the inhibitory effects of ophiopogonin-B （OP-B ） on cell adhesion,invasion and migration in non-small cell lung cancer （NSCLC） A549 cells in vitro and its possible mechanism.Meth-ods Cell adhesion assay and transwell chamber assay were used to detect the ability of cell adhesion,migra-tion and invasion.qRT-PCR was used to detect the mRNA levels of MMP-2 and 9 .Meanwhile ,Western＆nbsp;blot assay was performed to determine the protein levels of MMP-2/9 and p-Akt.Results OP-B significantly inhibited the ability of cell adhesion,invasion and mi-gration in A549 cells at the concentration of 10 μmol· L-1 （P〈0.01 ）.Meanwhile,it inhibited the mRNA and protein levels of MMP-2 and MMP-9 and down-regulated the phosphorylation of Akt （P 〈0.0 1 ）.＆nbsp;Conclusion OP-B inhibits cell adhesion,invasion and migration in A549 cells through down-regulation of the mRNA and protein expression of MMP-2/9 ,and the inhibitory effect on the expression of p-Akt.

关 键 词：麦冬皂苷B 非小细胞肺癌 黏附 侵袭 迁移 基质金属蛋白酶-2 基质金属蛋白酶-9 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]