激光捕获显微切割技术纯化的乳腺癌上皮细胞和间质比较蛋白质组学研究  

Comparative proteome research of epithelial cells and stroma from invasive ductal carcionma patients

作　　者：徐学清[1] 刘叔文[1] 

机构地区：[1]南方医科大学药学院,广东广州510515

出　　处：《中国药理学通报》2015年第5期697-703,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 31370782);广东省优秀青年教师培养计划项目资助

摘　　要：目的研究乳腺癌上皮细胞和间质间蛋白表达的差异。方法用改良HE染色法对临床侵润性导管内原位癌患者的石蜡包埋样品进行染色,用激光捕获显微切割技术(LCM)纯化分离癌变的乳腺上皮细胞和其周围间质后分别进行质谱分析。结果质谱总共检测了431种蛋白,上皮细胞和间质中分别检测到384和298种蛋白。251种共同表达的蛋白中,69种间质蛋白的含量比在上皮细胞中高,60种比在上皮细胞中低。所检测到蛋白的上皮细胞和间质定位及表达水平和它们在肿瘤发生、发展过程中的作用一致。结论在上皮细胞和间质中差异表达蛋白可以是疾病的筛选、诊断和预后判断的蛋白标志。Aim To study the difference of proteins expression of epithelial cells and stroma of breast canc-er patients.Methods Formalin-fixed paraffin-embed-ded tissue samples from invasive ductal carcionma pa-tients were stained by improved HE staining methods. And the neoplastic epithelium and stroma were micro-dissected using laser capture microdissection and ana-lysed by mass spectrometry.Results Total 43 1 differ-ent proteins were detected by mass spectrometry and there were respectively 384 and 298 different kinds of proteins identified in epithelial cells and stroma.A-mong them,25 1 proteins were commonly expressed in two samples,while the contents of 69 and 60 proteins＆nbsp;in stroma were respectively higher or lower than the ones in epithelial cells.The expression level and local-ization of proteins identified in epithelial cells and stro-ma were associated with their roles in development and progression of tumor cells.Conclusions The differen-tial expression proteins between epithelial cells and stroma of invasive ductal carcionma patients may func-tion as biomarkers for breast cancer screenning,diag-nosis and prognosis.

关 键 词：乳腺癌 肿瘤微环境 间质 激光捕获显微切割 乳腺上皮细胞 质谱 

分 类 号：R341[医药卫生—基础医学] R394.3