内吗啡肽-1对人树突状细胞TLR2和TLR4表达的影响  

Effect of endomorphin-1 on TLR2 and TLR4 expressions of dendritic cells from human blood

作　　者：杨丽娟[1] 潘治宇 陈勇[2] 陶志勇[2] 夏惠[2] 李正红[1] 

机构地区：[1]蚌埠医学院生理学教研室,安徽蚌埠233030 [2]蚌埠医学院安徽省感染与免疫重点实验室,安徽蚌埠233030

出　　处：《中国药理学通报》2015年第5期704-708,共5页Chinese Pharmacological Bulletin

基　　金：安徽省教育厅自然科学研究重点项目(No KJ2011A202);蚌埠医学院科技发展基金重点项目(No Bykf13A10)

摘　　要：目的观察内吗啡肽-1(EM-1)对人外周血来源树突状细胞TLR2和TLR4表达的影响。方法从人外周血中提取和分离单核细胞,在含重组人粒-巨噬细胞集落刺激因子和重组人白细胞介素-4的完全培养基中培养,6 d后未成熟树突状细胞(im DC)分4组,空白对照组(BLA组)、EM-1组、LPS组、LPS+EM-1组。继续培养2 d后,流式细胞术检测各组DC表面TLR2、TLR4蛋白的表达;RT-PCR法检测各组DC中TLR2 mRNA、TLR4 mRNA的表达。结果流式结果显示,TLR2、TLR 4在im DC表达较高,随着DC成熟表达下降。与BLA组相比,EM-1组DC表面TLR2、TLR4的表达下调(P<0.05);与LPS组相比,LPS+EM-1组DC表面TLR2、TLR4受体均明显下调(P<0.01)。RT-PCR的结果显示,与BLA组相比,EM-1干预后引起DC表面TLR2 mRNA表达明显降低(P<0.01),TLR4 mRNA的变化无差异(P>0.05);与LPS组相比,LPS+EM-1组DC表面TLR2 mRNA、TLR4mRNA均有所下调(P<0.05)。结论 EM-1使DC表面TLR2、TLR4的表达下调,EM-1对DC免疫功能的影响可能与DC表面TLR2、TLR4表达有关。Aim To observe the effect of endomorphin-1 （EM-1 ）on TLR2 and TLR4 expressions of dendritic cells （DC）from human peripheral blood.Methods Monocytes isolated from human peripheral blood mono-nuclear cells were cultured in medium containing re-combinant human interleukin-4 and recombinant hu-man granulocyte macrophage colony stimulating factor. After six days of culture,the immature dendritic cells （imDC ） were divided into four groups,the control group （BLA group）,EM-1 group,LPS group and LPS＋EM-1 group.After 2 days of culture,the expres-sions of TLR2 and TLR4 were determined by fluores-cence activated cell sorter（FACS）.The expressions of TLR2 and TLR4 at mRNA level in DC were detected by RT-PCR.Results The FACS results showed that the expressions of TLR2 and TLR4 in imDC were high-er,and their expressions were decreased with the mat-uration of DC.Compared with BLA group,the expres-sions of TLR2 and TLR4 in DC were down-regulated in EM-1 group （P0.05 ）.mR-NA expressions of TLR2 and TLR4 on DC in LPS ＋EM-1 group were lower than those in LPS group （P〈0.05 ）.Conclusions EM-1 enables the down-regula-tion of the expressions of TLR2 and TLR4 on DC sur-face,the effects of EM-1 on immune function may be associated with TLR2 and TLR4 expressions on DC surface.

关 键 词：内吗啡肽-1 树突状细胞 TLR2 TLR4 免疫功能 抗原提呈细胞 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R341.6[医药卫生—基础医学]