不同环境温度下P2X5受体表达与虚寒证、虚热证关系的研究  

The Relationship Study between Expressions of P2X5 Receptor and Deficiency-cold Syndrome / Deficiency-heat Syn-drome at Various Ambient Temperatures

作　　者：杨丽萍[1] 于洪洁[1] 黄睿[1] 李新民[1] 詹向红[1] 侯俊林[1] 

机构地区：[1]河南中医学院中西医结合基础学科,郑州450046

出　　处：《中国中西医结合杂志》2015年第5期559-562,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81072734);国家重点基础研究发展计划(No.2011CB505102)

摘　　要：目的检测在不同温度环境下外周血中的P2X5受体的表达情况，探讨其与虚寒证、虚热证之间的关系。方法通过量表及专家诊断筛选出正常组、虚寒证组及虚热证组各20例，抽取室温（25℃）及低温（－4～5℃）下受试人员肘静脉血样5mL，用实时荧光定量PCR对P2X5受体表达进行相对定量，分别比较3组受试人员室温和低温下P2X5受体的表达情况。结果在室温状态下，虚寒证、虚热证组P2X5受体表达低于正常组（P〈0.05）；在低温状态下，与正常组比较，虚寒证组P2X5受体表达降低更明显（P〈0.01），且低于虚热证组，差异有统计学意义（P〈0.05），3组组内P2X5受体在两种温度下的表达均无差异（P〉0.05）。结论不同的中医证候在不同的外界环境温度下P2X5受体的表达情况不同。外界环境温度对同一证候人群的P2X5受体表达的影响不显著。Objective To detect the expression of the peripheral blood P2X5 receptor at various ambient temperatures, and to explore its relationship with deficiency-cold syndrome and deficiency-heat syndrome. Methods Subjects were selected by questionnaire and expert diagnosis, and assigned to the normal control group, the deficiency-cold syndrome group, and the deficiency-heat syndrome group, 20 in each group. 5 mL venous blood was collected at room temperature （25℃） and cold temperature （-4 -5℃） respectively. Then the expression of P2X5 receptor was relatively quantified by real-time fluorescence quantitative PCR, and compared at room temperature and cold temperature respectively. Results The expression of P2X5 receptor in deficiency-cold syndrome and deficiency-heat syndrome groups was lower than that in the normal control group at room temperature （P 〈0.05）. It decreased more at cold temperature in the deficiency-cold syndrome group than in the normal control group （P 〈 0.01） as well as in the deficiency-heat syndrome group （P 〈0.05）. The expression of P2X5 receptor showed no difference in all groups at two different temperatures （P 〉0.05）. Conclusions The expression of P2X5 receptor was different in different syndrome groups at various ambient temperatures. Ambient temperatures had insignificant effect on the expression of P2X5 receptor of the population with the same syndrome.

关 键 词：实时荧光定量PCR P2X5受体 虚寒证 虚热证 

分 类 号：R241[医药卫生—中医诊断学]