基因芯片表达分析4Gyγ射线对小鼠骨髓c-kit阳性细胞影响  

Expression changes of genes in c-kit positive cells after 4 Gy γ-ray irradiation： a gene chip analysis

作　　者：张俊伶[1] 路璐[1] 李德冠[1] 孟爱民[1] 

机构地区：[1]天津市放射医学与分子核医学重点实验室,中国医学科学院放射医学研究所,300192

出　　处：《国际放射医学核医学杂志》2015年第2期116-120,共5页International Journal of Radiation Medicine and Nuclear Medicine

基　　金：国家自然科学基金海外合作基金项目（81129020）;国家重点基础研究发展计划（“973”项目）重大专项（2011CB964800-G）;国家自然科学基金面上项目（81372928）;国家自然科学基金青年项目（81402633）;协和青年基金资助和中央高校基本科研业务费专项资金资助项目（33320140033）

摘　　要：目的 利用基因芯片技术分析经4 Gyγ射线照射后c-kit（CD117,正常表达于干祖细胞表面的细胞因子受体）阳性细胞与代谢过程相关的基因及信号通路表达变化.方法 利用磁珠分选系统分选出骨髓c-kit阳性细胞.实验分为对照组、4 Gy照射组.取1×10^6个c-kit阳性细胞进行照射,照射剂量率为0.99 Gy/min.照射后将细胞培养18h后取出,进行全基因组的高通量基因芯片检测,并进行Gene Ontology聚类分析和Kyoto Encyclopedia of Genes and Genomes信号通路分析.结果 4Gyγ射线照射引起c-kit阳性细胞包括Pld5、Neu2、Bpgm、Alas2、Satl1、Rdh16、Mccc1、Sat2、Smug1、Cml5、Adhfe1、Idh3a、Slc27a5在内的13个基因表达上调3倍以上,包括Acss2、Phgdh、Psat1、Glb1、Gpam、Dus3l、Fuca2、Impdh1、Dlat、Glb1、Enpp4、Prim2、Mettl10、Slc27a2、Dera、Qdpr、Dus1l、Cdyl2、Dhodh、Srr、Spr、Mical2在内的22个基因表达下调3倍以上,这些基因主要参与嘌呤代谢、嘧啶代谢、三羧酸循环等信号通路.结论 4Gyγ射线照射能引起c-kit阳性细胞中与代谢过程相关的许多基因表达发生变化,这些变化的基因有待进一步的实验验证.Objective To analyze the metabolic process-related genes and pathway variations in the c-kit （CD 117,a cytokine receptor expressed on the surface of hematopoietic stem cells） positive cells after 4 Gy irradiation by using gene chip technology.Methods c-kit positive cells were sorted by microbead separation system.Two groups were involved：control group and 4 Gy irradiation group.A total of 1 ×10^6 c-kit positive cells received 4 Gy irradiation at a dose rate of 0.99 Gy/min.After the two groups were cultured for 18 h,all the cells were collected and detected by gene chip arrays.Data were processed and analyzed via Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways.Results After 4 Gy γ-ray irradiation,13 metabolic process-related genes were up-regulated more than 3 times：Pld5,Neu2,Bpgm,Alas2,Satl1,Rdh16,Mccc1,Sat2,Smug1,Cml5,Adhfe1,Idh3a,and Slc27a5.Additionally,22 genes were down-regulated more than 3 times：Acss2,Phgdh,Psat1,Glb1,Gpam,Dus3l,Fuca2,Impdh1,Dlat,Glb1,Enpp4,Prim2,Mettl10,Slc27a2,Dera,Qdpr,Dus1l,Cdyl2,Dhodh,Srr,Spr,and Mical2.These genes were involved in pyrimidine metabolism,purine metabolism,and citrate cycle （TCA cycle） pathways.Conclusion Many variations of genes and pathways in c-kit positive cells existed after 4 Gy irradiation.However,further laboratory technology is required to confirm these variations.

关 键 词：原癌基因蛋白质C-KIT 寡核苷酸序列分析 辐射 电离 细胞代谢 

分 类 号：R818[医药卫生—放射医学]