丙氨酰-谷氨酰胺对低出生体重新生大鼠缺氧性肠损伤后胰岛素样生长因子1的影响  

Effect of L-alanyl-L-glutamine on expression of insulin-like growth factor-1 in intestinal tissues of low-birth-weight newborn rats with hypoxia/reoxygenationinduced intestinal injury

作　　者：徐芬[1] 朱传瑞[1] 詹媛丽 卢光进[1] 苏浩彬[2] 

机构地区：[1]深圳市宝安区妇幼保健院新生儿科,广东深圳518133 [2]中山大学孙逸仙纪念医院儿科,广州510120

出　　处：《中国当代儿科杂志》2015年第5期502-507,共6页Chinese Journal of Contemporary Pediatrics

摘　　要：目的探讨丙氨酰-谷氨酰胺(Ala-Gln)对低出生体重(LBW)新生大鼠缺氧后肠组织胰岛素样生长因子-1(IGF-1)及IGF-1受体(IGF-1R)水平的影响。方法将孕鼠分别置于吸烟和非吸烟环境中饲养,非吸烟条件下饲养孕鼠所分娩的新生大鼠设为A组;将吸烟条件下饲养孕鼠所分娩的正常体重新生大鼠设为B组,LBW新生大鼠随机分为对照组(C组)、缺氧-复氧组(D组)和Ala-Gln组(E组);每组24只。D、E组行缺氧-复氧操作连续3d,每天2次造模,E组在每日缺氧处理前腹腔注射Ala-Gln(10m L/kg),C、D组以等量生理盐水行替代注射。各组于生后第4、7、10天分别处死8只大鼠取肠组织。采用ELISA法检测各组肠组织IGF-1水平;免疫组化法检测各组肠组织IGF-1R水平。结果 A组与B组IGF-1及IGF-1R蛋白水平在各时间点差异无统计学意义(P>0.05)。C组IGF-1及IGF-1R蛋白水平呈上升趋势,第7天时均较其他各组升高(P<0.05),第10天时趋于正常水平,与A、B组比较差异无统计学意义(P>0.05)。D组各时间点IGF-1及IGF-1R蛋白水平均明显低于C组(P<0.05)。E组在第4、7天IGF-1及IGF-1R蛋白水平低于C组(P<0.05),但到第10天上升至接近C组水平,且显著高于D组水平(P>0.05)。结论宫内和生后缺氧易使LBW新生大鼠出现肠损伤,肠道外给予大剂量Ala-Gln可减轻LBW新生大鼠缺氧性肠损伤程度,对缺氧性肠损伤有一定保护作用。ObjectiveTo study the effect of L-alanyl-L-glutamine （Ala-Gln） on the levels of insulin-like growth factor-1 （IGF-1） and IGF-1 receptor （IGF-1R） in the intestinal tissues of low-birth-weight （LBW） newborn rats with hypoxia/reoxygenation-induced intestinal injury.MethodsPregnant rats were fed with or without smoking. The rats born by those fed without smoking were included in group A; for the rats born by those fed with smoking, normal-birth-weight rats were included in group B, and LBW rats were randomly divided into control group （group C）, hypoxia/reoxygenation （H/R） group （group D）, and Ala-Gln group （group E）. Each group consisted of 24 newborn rats. The rats in groups D and E received H/R treatment twice a day for three consecutive days to establish an intestinal injury model; the rats in group E were intraperitoneally injected with Ala-Gln （10 ml/kg） before daily H/R treatment, while those in groups C and D were given an equal dose of normal saline by intraperitoneal injections. On days 4, 7, and 10 after birth, 8 rats were sacrificed in each group to collect intestinal tissues. The IGF-1 levels in intestinal tissues were measured using ELISA, and IGF-1R levels were measured by immunohistochemistry.ResultsThere were no signiifcant differences in IGF-1 and IGF-1R levels between groups A and B at all time points. The levels of IGF-1 and 〈br〉 IGF-1R in group C kept increasing, were higher than those in other groups on day 7 （P〈0.05）, and reached a normal level on day 10, without signiifcant differences compared with those in groups A and B. Group D had signiifcantly lower IGF-1 and IGF-1R levels than group C at all time points （P〈0.05）. The levels of IGF-1 and IGF-1R in group E were lower than those in group C on days 4 and 7 （P〈0.05）, but they increased to approximately the levels in group C and were signiifcantly higher than those in group D on day 10.ConclusionsIntrauterine and postnatal hypoxia may induce intestinal injury in LBW newborn rats, and p

关 键 词：丙氨酰-谷氨酰胺 低出生体重 胰岛素样生长因子-1 肠损伤 新生大鼠 

分 类 号：R722.1[医药卫生—儿科]