广西钦州湾红树林抗菌活性菌株Erwinia sp.5-8的筛选及发酵条件的优化  

Screening the Antimicrobial Bacterium Strain of Erwinia sp.5-8 From Guangxi Mangrove and Optimazing its Fermentation Conditions

作　　者：孟令洋[1,2] 姜伟[2,3] 张培玉[1] 林学政[2,3] 

机构地区：[1]青岛大学环境科学与工程学院,山东青岛266071 [2]国家海洋局第一海洋研究所,山东青岛266061 [3]国家海洋局海洋生物活性物质重点实验室,山东青岛266061

出　　处：《海洋科学进展》2015年第2期270-278,共9页Advances in Marine Science

基　　金：海洋公益性行业科研专项经费项目--海洋微生物小分子化合物资源库构建及药源活性物质的筛选应用(201005022-6)

摘　　要：以金黄色葡萄球菌为指示菌,采用琼脂扩散法从国家海洋局海洋生物活性物质重点实验室广西钦州湾红树林微生物资源库中筛选获得一株具有明显抑菌效果的菌株5-8,对该菌株进行了分子鉴定与系统发育分析。结果表明,此菌为欧文氏菌属(Erwinia)。通过单因子试验对其发酵条件进行了优化,优化后发酵条件为:最佳氮源为蛋白胨,最佳碳源为葡萄糖,发酵培养基初始pH值为8.0,发酵温度为25℃,盐度为1.5,摇瓶装液量30%,接种量为1%(V种子液∶V培养液=1∶100)。In this study,an antimicrobial bacterium strain, named 5-8, was screened and isolated from man- grove of Guang Xi by the method of agar diffusion using Staphylococcus aureu as the indicator strain. The results of molecular identification and phylogenetic analysis showed that this strain belonged to genera of Erwinia. The single factor experiments on the fermentation conditions were conducted and the optimized parameters were as follows： the best nitrogen source is peptone, the best carbon source is glucose , the starting pH of medium was 8.0,fermentation temperature was 25℃ ,salinity was 1.5%, liquid volume in erlenmeyer flask was 30 % and inoculation amount was 1 % （Vseed liquid/Vnutri lutio = 1 ： 100）.

关 键 词：红树林 欧文氏菌 抗菌活性 系统发育分析 发酵条件优化 

分 类 号：Q939.9[生物学—微生物学]