天麻素对心肌缺血再灌注损伤大鼠血清内皮素-1水平的影响  

Effect of gastrodin on the level of endothelin-1 in rats with myocardial ischemia-reperfusion injury

作　　者：哈玲梅[1] 董化江[2] 单娜娜[3] 臧照辉[4] 何文彤[2] 

机构地区：[1]武警后勤学院附属医院急诊科,天津300162 [2]武警后勤学院人体解剖学与组织胚胎学教研室天津市遗体防腐与整形整容重点实验室,天津300162 [3]武警后勤学院教育技术中心,天津300162 [4]武警后勤学院研究生处,天津300162

出　　处：《新乡医学院学报》2015年第5期395-397,共3页Journal of Xinxiang Medical University

基　　金：国家十一五科技支撑计划项目(编号:2007BAK38B05);武警后勤学院科研创新团队项目(编号:WHTD201308-2)

摘　　要：目的探讨天麻素对心肌缺血再灌注损伤大鼠血清中内皮素-1(ET-1)水平的影响。方法 Wistar大鼠50只随机分为5组:假手术组、模型组、天麻素低剂量组、天麻素中剂量组、天麻素高剂量组,每组10只。假手术组大鼠开胸不结扎冠状动脉前降支;模型组大鼠开胸结扎前降支;天麻素低、中、高剂量组大鼠开胸结扎冠状动脉前降支,术前给予相应剂量的天麻素,经舌下静脉给药。放射免疫法测量ET-1血清学水平;反转录-聚合酶链反应、蛋白质免疫印迹法检测各组大鼠心肌组织中ET-1的表达情况。结果与假手术组比较,模型组及天麻素低、中、高剂量组大鼠血清ET-1水平、心肌组织ET-1 mRNA及蛋白水平显著升高(P<0.05);天麻素低、中、高剂量组大鼠血清ET-1水平、心肌组织ET-1 mRNA及蛋白水平均较模型组显著降低(P<0.05),且天麻素剂量越高其水平降低越明显(P<0.05)。结论天麻素进行干预后可有效降低心肌缺血再灌注损伤后内皮素-1表达。Objective To explore the effect of gastrodin on the level of endothelin-1 in rats with myocardial ischemia- reperfusion injury. Methods Fifty Wistar rats were randomly divided into sham operation group, model group,low dose of gastro- din group,middle dose of gastrodin group and high dose of gastrodin group, 10 rats in each group. The rats in sham operation group were opened thoracic cavity without ligating anterior descending coronary, the rats in model group were opened thoracic cavity and ligated anterior descending coronary,while the rats in low,middle and high dose of gastrodin group were given corre- sponding dose of gastrodin by the sublingual vein before opening thoracic cavity and ligating anterior descending coronary. The level of serum endnthelin-I was detected by radioimmunoassay and the endotbelin-1 in cardiac muscle tissue was detected by re- verse transcription polymerase chain reaction and Western blot methods. Results Compared with sham operation group,the level of serum endothelin-1 ,endothelin-1 mRNA and endothelin-1 protein in cardiac muscle tissue were significantly higher in model group and low,middle and high dose of gastrodin group（P 〈0.05）. The level of serum endothelin-1 ,endothelin-1 mRNA and en- dothelin-1 protein in cardiac muscle tissue in low,middle and high dose of gastrodin group were significantly lower than those in model group（ P 〈0.05 ）, and with the dose of gastrodin increasing,the reducing extent was more obvious obvious （P 〈 0.05 ）. Conclusion Gastrodin can reduce the expression of endothelin-1 in myocardial ischemia-reperfusion injury in rats effectively.

关 键 词：天麻素 大鼠 心肌缺血再灌注损伤 内皮素-1 

分 类 号：R542.2[医药卫生—心血管疾病]