Rep78与PML相互作用并抑制HSV-1的增殖（英文）  

Rep78Interacts with PML and Inhibits the Infection of HSV-1

作　　者：宋玏笙 王然[1] 卢韦[1] 谢林俊[1,2] 王浩[1] 薛京伦[1] 陈金中[1] 

机构地区：[1]复旦大学生命科学学院遗传工程国家重点实验室,上海200433 [2]扬州大学药学院药学系,扬州225001

出　　处：《复旦学报（自然科学版）》2015年第2期245-251,共7页Journal of Fudan University：Natural Science

基　　金：Project supported by National Basic Research Program of China(2010CB529903);the National Natural Science Foundation of China(31071171,81170532)

摘　　要：AAV-2Rep78蛋白是涉及到病毒转录、复制和位点特异性整合的多功能蛋白,它同时还介导AAV-2与其协助病毒之间的相互作用.我们通过免疫共沉淀实验证明细胞内PML蛋白与Rep78蛋白存在相互作用,作用区域位于Rep78蛋白C末端545FPCRQCERM553位置,在细胞内短暂表达Rep78会加速PML蛋白的降解.分别使用含有ICP0的病毒株G207和ICP0突变的病毒株7134感染P5-Rep78转基因Vero细胞系,结果表明Rep78的表达会抑制G207的增殖但是对ICP0突变的7134病毒没有明显的抑制作用.结果表明Rep不仅可以介导定点整合,同时也是AAV协助病毒的负调控物.AAV-2 Rep78 is a multifunctional protein required for viral transcription, replication, and site-specificintegration. It also mediates the interaction between AAV-2 and its helper virus. We identified the cellular proteinPML as a Rep78 interacting protein by co-immunoprecipitatioru Further, based on the hybridization assay with anoverlapped array of Rep protein and HEK293 cell lysate, the interacting fragment was located at545FPCRQCERM553 of Rep78 specific C terminus. Transient expression of Rep？8 resulted in an increaseddegradation of cellular PML, and the degradation could be partially recovered by treatment with MG132. Here,the HSV-1 strain G207 containing ICP0 and an ICP0-null mutant HSV-1 were transfected in a P5-rep78 transgeneVero cell line, respectively, we observed an inhibitory effect of Rep78 expression on wide-type HSV-1 replication,but not with the ICP0-null viral mutant HSV-1. The results suggested that Rep is not only a target, but also acontrary regulator for the helper virus of AAV.

关 键 词：Ⅰ型单纯疱疹病毒 REP蛋白 早幼粒细胞白血病蛋白 降解 Ⅰ型单纯疱疹病毒早早期蛋白ICP0 

分 类 号：Q782[生物学—分子生物学]