基于金-石墨烯修饰的高灵敏甲胎蛋白电化学免疫传感器  

Highly sensitive electrochemical immunosensor for α-1-fetoprotein based on gold-graphene nanocomposite

作　　者：朱强[1,2] 余红霞[2] 

机构地区：[1]重庆师范大学化学学院绿色合成与应用重点实验室,重庆401331 [2]西南大学化学化工学院,重庆400715

出　　处：《化学传感器》2015年第1期47-51,共5页Chemical Sensors

基　　金：重庆市高校创新团队资助(KJTD201309);重庆市教委科学技术项目(KJ130640)

摘　　要：以具有大比表面积和良好生物相容性的纳米金颗粒（Au NPs）和金-石墨烯复合物（Au-Gra）为基底,成功制备了高灵敏甲胎蛋白免疫传感器。首先在金电极表面电沉积一层纳米金,接着滴涂一层具有独特电化学活性的铁氰化镍纳米粒子（Ni NPs）电子媒介体,然后滴加一层Au-Gra复合膜,最后利用Au-Gra结合甲胎蛋白抗体（anti-AFP）,从而制得甲胎蛋白免疫传感器。采用循环伏安法对电极性能进行了验证,结果表明该传感器有良好的电流响应性能,检测线性范围为0.1~100 ng/m L,检出限为0.03 ng/m L（S/N=3）。此传感器简单、快速、灵敏,可望用于临床诊断。Based on gold nanparticles（Au NPs） and gold-grephene（Au-Gra） nanomaterials, an highly sensitive amprometric immunosensor for α-1-fetoprotein was successfully prepared. Firstly, Au NPs were electrodeposited on the surface of bare gold electrode. Then Ni NPs were dropped on the electrode and served as electrochemical redox probe, next Au-Gra was fabricated to combine anti-AFP. The electrochemical characteristics of the immunosensor were demonstrated by cyclic voltammetry（CV）. The immunosensor exhibited a good linear range from 0.1 to 100ng/m L with a detection limit of 0.03 ng/m L（S/N=3）. The proposed immunosensor was simple, rapid and sensitive,and may provide a promise method for clinical detection. of AFP

关 键 词：免疫传感器 金-石墨烯 铁氰化镍 甲胎蛋白 

分 类 号：O657.1[理学—分析化学] TP212[理学—化学]