A bestatin-based fluorescent probe for aminopeptidase N cell imaging  

作　　者：Hui Chen Hui Wang Xiao-Jun Qin Chen Chen Lu Feng Lai-Zhong Chen Lu-Pei Du Min-Yong Li 

机构地区：[1]Department of Medicinal Chemistry,Key Laboratory of Chemical Biology(MOE),School of Pharmacy,Shandong University [2]China Pharmaceutical University Pharmaceutical Co.Ltd.

出　　处：《Chinese Chemical Letters》2015年第5期513-516,共4页中国化学快报（英文版）

基　　金：supported by the Program for New Century Excellent Talents in University(No.NCET-11-0306);the Shandong Natural Science Foundation(No.JQ201019);the Independent Innovation Foundation of Shandong University, IIFSDU(No.2014JC008);the Graduate Independent Innovation Foundation of Shandong University,GIIFSDU(No.yzcl2096)

摘　　要：Aminopeptidase N （APN） is an important drug target and biomarker for various tumors. The current work characterizes a novel APN-targeted fluorescent probe （Bes-Green, 2） that manifests comparable inhibitory activity with Bestatin. This probe has capacity of tightly binding to the APN for imaging endogenous APN in living human ovarian clear cell carcinoma cells （ES-2） and has potential application in biological study of cellular APN.Aminopeptidase N （APN） is an important drug target and biomarker for various tumors. The current work characterizes a novel APN-targeted fluorescent probe （Bes-Green, 2） that manifests comparable inhibitory activity with Bestatin. This probe has capacity of tightly binding to the APN for imaging endogenous APN in living human ovarian clear cell carcinoma cells （ES-2） and has potential application in biological study of cellular APN.

关 键 词：Aminopeptidase N Bestatin Cell imaging Fluorescent probe 

分 类 号：R96[医药卫生—药理学] O657.3[医药卫生—药学]