RAW264.7细胞分化为破骨细胞的凋亡观察  

Investigation of the apoptosis of osteoclast-like-cells differentiated from RAW264.7cells

作　　者：付应霄[1,2] 顾建红[1] 王怡[1] 袁燕[1] 刘学忠[1] 卞建春 刘宗平[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]蚌埠医学院生物科学系,安徽蚌埠233030

出　　处：《扬州大学学报（农业与生命科学版）》2015年第1期6-10,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(31172373;31372495;31302154);江苏省高校优势学科建设工程项目(2010-10);江苏省动物重要疫病与人兽共患病防控协同创新中心课题(2013-10);教育部博士点基金项目(20113250110003);安徽省自然科学基金资助项目(1508085QH172)

摘　　要：采用M-csF＋RANKL联合诱导RAW264．7细胞分化为破骨样细胞（OCL），观察培养过程中的凋亡现象，了解其生长规律。在诱导培养的第5～9天，通过TRAP染色、检测TRAP基因表达水平、细胞骨架与胞核染色方法分析OCL凋亡状况。结果表明：体外培养成熟OCL短时间内即发生凋亡，表现为TRAP阳性细胞数减少，TRAP基因表达水平降低，细胞骨架结构破坏、胞核形态改变。Osteoclast-like-cells （OCLs） were induced from RAW264.7 via M-CSF＋ RANKL, the apoptosis of OCLs were investigated, and the growth rhythm of OCLs were observed. TRAP staining, TRAP gene expression level assay, cytoskeleton and nuclei staining were employed to study the apoptosis of OCL from 5th to 9th d. The results indicated that mature OCL apoptosis developed in a short term in vitro. The apoptotic OCL was characterized by decline in TRAP- positive cells number, reduction in TRAP gene expression level, destruction of cytoskeleton and morphological changes of nuclei.

关 键 词：RAW264.7细胞 破骨样细胞 凋亡 

分 类 号：Q813.1[生物学—生物工程]