地衣芽孢杆菌W10菌株发酵培养基优化  

Optimization of medium for the fermentation of Bacillus licheniformis W10

作　　者：宋浩[1,2] 纪兆林[1] 陈夕军[1] 徐敬友[1] 童蕴慧[1] 

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009 [2]沭阳县农业科学研究所,江苏沭阳223600

出　　处：《扬州大学学报（农业与生命科学版）》2015年第1期87-91,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：现代农业产业技术体系建设专项(CARS-31-2-02)

摘　　要：在培养基单因子筛选试验基础上,采用响应曲面法对地衣芽孢杆菌W10菌株发酵培养基组成进行优化。结果表明:最佳发酵培养基配方(g·L-1)为黄豆饼粉10.13、玉米淀粉16.89、蛋白胨1.13、葡萄糖2.41、硫酸铵7.96、硫酸镁0.45、磷酸氢二钾0.45、氯化钠4.50。用优化培养基对W10菌株进行发酵培养,发酵液对灰葡萄孢的抑菌率为90.37%,比优化前抑菌率(73.90%)提高22.3%。Bacillus licheniformis Wl0, an antifungal bacterium strain, possessed strong inhibiting effect on Botrytis cinerea and other important plant pathogens. The medium for the fermentation of B. licheniformis Wl0 was optimized by single factor analysis and respond surface methodology. The results showed that the optimum fermentation medium of Wl0 strain consisted of 10. 13 g·L^1 1 soybean flour, 16.89 g·L^-1 corn flour, 1.13 g· L-1 peptone, 2.41 g· L^-1 glu- cose, 7.96 g· L^- 1（NH4）2SO4, 0.45 g· L^- 1 MgSO4, 0.45 g·L ^-1 K2HPO4, and 4.50 g·L-1 NaC1. The inhibitory effect of the W10 strain using the optimized culture medium on B. cinerea reached 90.37%, showing a 22.3% increase compared to 73. 900%o before medium optimization.

关 键 词：地衣芽孢杆菌 培养基优化 响应曲面法 

分 类 号：S476[农业科学—农业昆虫与害虫防治]