银杏雄株ISSR分子标记及亲缘关系分析  

Genetic identification and phylogenetic analysis of Ginkgo biloba male plants using ISSR molecular marker

作　　者：李卫星[1] 花艳敏 张秀萍[2] 陈鹏[1] 

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009 [2]南通大学纺织服装学院,江苏南通226019

出　　处：《扬州大学学报（农业与生命科学版）》2015年第1期101-106,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏省林业三新工程项目(LYSX[2014]12)

摘　　要：采用ISSR分子标记对42个银杏雄株进行亲缘关系分析。从100条引物中筛选出12条多态性引物,共扩增得到94条带,多态性条带56条,平均每个引物扩增的多态性带数为7.83条,多态性百分率为59.57%,表明银杏雄株的遗传多样性较高。用UPGMA方法进行聚类分析,在遗传相似系数为0.51水平上将42个银杏雄株分为3个类群。用EIGEN方法进行主坐标分析,建立亲缘关系的三维图。两种分类方法所获结果基本一致,雄株的亲缘关系与地区分布不完全相关,山东与江苏的部分银杏雄株试材亲缘关系较近。ISSR molecular markers were used for phylogenetic analysis among 42 male plants of Ginkgo biloba. 12 polymorphic primers were screened from 100 primers, and a total number of 94 bands were amplified using these prim- ers, among which 56 bands were polymorphic and average bands for each primer pair were 7.83, and the polymorphism ratio was 59.57%. The above results suggested that the genetic diversity was higher among Ginkgo males. Cluster analy- sis was performed by the method of UPGMA, and 42 male ginkgo plants were divided into 3 groups at the genetic similar- ity coefficient level of 0.51. A three-dimensional map representing relationships among male plants was also established by principal coordinate analysis with the EIGEN method. The two kinds of classification methods above exhibited similar results that the phylogenetic relationship was not completely correlated with regional distribution. Genetic relationship of partial male plants between Shandong and Jiangsu were relatively closer.

关 键 词：银杏 雄株 亲缘关系 简单重复序列(ISSR) 聚类分析 

分 类 号：S664.3[农业科学—果树学]