气体二氧化氯快速制备技术及在生物安全柜消毒中的应用研究  

Study on rapid preparation of gaseous chlorine dioxide and its application in biosafety cabinet disinfection

作　　者：郝丽梅[1] 吴金辉[1] 王涛[1] 林松[1] 衣颖[1] 徐廷昕 祁建城[1] 

机构地区：[1]军事医学科学院卫生装备研究所国家生物防护装备工程技术研究中心,天津300161 [2]北京万杰朗医疗技术有限公司

出　　处：《中国消毒学杂志》2015年第6期550-553,共4页Chinese Journal of Disinfection

基　　金：艾滋病和病毒性肝炎等重大传染病防治"国家科技重大专项(No.2012ZX10004401; 2012ZX10004801003)

摘　　要：目的 研究气体二氧化氯快速制备技术,建立生物安全柜快速消毒方法。方法 利用酸性激活原理快速制备二氧化氯气体,采用载体定性杀菌试验方法观察二氧化氯气体杀菌效果。结果 将亚氯酸盐与有机酸盐以2∶1比例组成A、B剂,通过专用消毒机扩散,可形成二氧化氯气体对有限空间进行消毒。在常温常湿条件下,生物安全柜内二氧化氯气体持续维持在2.7～4.8mg/L,作用180min后可使生物安全柜工作区内及排风高效空气过滤器下游菌片上的枯草杆菌黑色变种芽孢达到全部杀灭。结论 本研究建立了现场快速制备气体二氧化氯的方法,并探索出生物安全柜有效消毒参数。Objective To study the rapid preparation method of gaseous chlorine dioxide so as to establish a rapid disin- fection method for biosafety cabinet. Methods Acid activation principle was used to produce gaseous chlorine dioxide. The carrier qualitative bactericidal test was used to estimate the germicidal efficacy of gaseous chlorine dioxide. Results Gase- ous chlorine dioxide, which prepared by mixing chlorite （reagent A） and organic acid salt （reagent B） with a mole ratio of 2 ： 1, could be diffused by a special disinfection machine and disinfect the limited space. At normal temperature and humidity conditions,Bacillus subtilis var. niger spores on the biosafety cabinet and the downstream of exhaust HEPA filter were abso- lutely sterilized after 180 min disinfection with the concentration of gaseous chlorine dioxide in the biosafety cabinet main- tain between 2. 7 mg/L to 4. 8 mg/L. Conclusion A rapid preparation method of gaseous chlorine dioxide is established, and the disinfection parameters of biological safety cabinet are also explored in this paper.

关 键 词：二氧化氯气体 快速制备 杀菌效果 生物安全柜消毒 

分 类 号：R187.2[医药卫生—流行病学]