HPLC法测定癃清片中表小檗碱、黄连碱、盐酸药根碱、盐酸巴马汀、盐酸小檗碱和丹皮酚  

Determination of epiberberine, coptisine, jatronhizine, palmatine, berberine, and paeonol in Longqing Tablets by HPLC

作　　者：张晓静[1] 刘德福[2] 丁菲菲[1] 田华[1] 邓雁如[1] 郭利平[3] 

机构地区：[1]天津中医药大学天津市中药化学与分析重点实验室,天津300193 [2]武警后勤学院附属医院药剂科,天津300162 [3]天津中医药大学附属保康医院,天津300193

出　　处：《现代药物与临床》2015年第5期527-530,共4页Drugs & Clinic

摘　　要：目的建立HPLC法同时测定癃清片中表小檗碱、黄连碱、盐酸药根碱、盐酸巴马汀、盐酸小檗碱、丹皮酚6种成分。方法 Waters Symmetry Shield RP18色谱柱（250 mm×4.6 mm,5μm）;流动相为乙腈（0.3%甲酸和0.3%三乙胺）–水（0.3%甲酸和0.3%三乙胺）,梯度洗脱;体积流量1.0 m L/min;检测波长270 nm;柱温25℃;进样体积20μL。结果表小檗碱、黄连碱、盐酸药根碱、盐酸巴马汀、盐酸小檗碱、丹皮酚分别在1.856~18.56、14.230~142.30、1.070~10.79、2.485~24.85、38.915~389.15、9.824~98.24μg/m L线性良好,r值均大于0.999 0。平均回收率分别为98.66%、99.49%、99.59%、98.66%、100.40%、99.95%,RSD值分别为2.91%、2.67%、2.65%、1.91%、3.42%、2.28%（n=9）。结论该方法专属性强,灵敏度高、重复性好、简单易行,可用于同时测定癃清片中表小檗碱、黄连碱、盐酸药根碱、盐酸巴马汀、盐酸小檗碱、丹皮酚6种成分,对完善癃清片的质量控制具有参考价值。Objectiwe To develop an HPLC method for simultaneous determination of epiberberine, coptisine, jatrorrhizin, palmatine, berberine, and paeonol in Longqing Tablets. Methods The analysis was performed on Waters Symmetry Shield TM RP18 column （250 mm x 4.6 mm, 5 lain） with cetonitrile （0.3% triethylamine and 0.3% phosphoric acid） - water （0.3% triethylamine and 0.3% phosphoric acid） as mobile phases for gradient elution. The flow rate was 1.0 mL/min. The detection wavelength was set at 270 nm. The column temperature was 25 ~C with injection volume of 20 pL. Results Epiberberine, coptisine, jatronhizine, palmatine, berberine, and paeonol had good linearity in the ranges of 1.856 -- 18.56, 14.230 -- 142.30, 1.070 -- 10.79, 2.485 -- 24.85, 38.915 -- 389.15, and 9.824 -- 98.24 Ixg/mL, respectively. The correlation coefficients of all curves were above 0.999 0. The average recoveries were 98.66%, 99.49%, 99.59%, 98.66%, I00.40%, and 99,95% with RSD 2.91%, 2.67%, 2.65%, 1.91%, 3.42%, and 2.28% （n=9）, respectively. Conclusion The method is specific, sensitive, repeatable, and simple for simultancous determinationg of epiberberine, coptisine, jatronhizine, palmatine, berberine and paeonol, which can be available for the quality control of Longqing Tablets.

关 键 词：癃清片 表小檗碱 黄连碱 盐酸药根碱 盐酸巴马汀 盐酸小檗碱 丹皮酚 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]