玉米生物诱导孤雌生殖DH群体的SNP偏分离分析  

Segregation Distortion study on Bio-induced Parthenogenesis-derived Doubled Haploid Lines of Maize by SNP

作　　者：许理文[1,2] 段民孝[2] 宋伟[2] 田红丽[2] 王凤格[2] 赵久然[2] 王守才[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]北京市农林科学院玉米研究中心,北京100097

出　　处：《玉米科学》2015年第3期8-14,共7页Journal of Maize Sciences

基　　金：北京市科技计划课题(D131100000213002);国家科技支撑项目(2011BAD35B09);北京市农林科学院科技创新能力建设专项(KJCX20140202);"863"计划"玉米产量功能基因研究与克隆"(2012AA10A305)

摘　　要：以单交种先玉335作母本,与京科诱006单倍体诱导系杂交,产生单倍体及秋水仙素加倍形成双单倍体(DH)群体,用SNP芯片对DH群体进行基因型分析,获得1 337个在先玉335的双亲间有多态的SNP数据,对SNP进行卡方检测(P<0.05),发现516个SNP表现偏分离,占38.6%,这些偏分离SNP中有285个偏向母本PH6WC,占55.2%;231个偏向父本PH4CV,占44.8%。在玉米的8条染色体上,发现10个偏分离热点区域(SDR),分析SNP的χ2值与其在染色体上的位置之间的关系发现,χ2值最大的SNP往往在SDR的中心位置。Haploids originating from the hybrid, Xianyu335（PH6WCxPH4CV） were crossed with the haploid in- ducer, Jingkeyou006 and the resulting doubled haploid lines（DHs） were obtained by chromosome doubling with eol- chieine. A total of 1337 SNP markers detected polymorphism between PH6WC and PH4CV. Among 1 337 SNP, cov- eting whole maize 10 chromosomes, five hundred and sixteen SNP（38.6%） showed the genetic distortion（P〈0.05）. Of the total segregation distortion SNP, two hundred and eighty-five SNP（55.2% ） deviated toward female parent, PH6WC, while 231 SNP（44.8%） deviated toward male parent, PH4CV. Totally, tensegregation distortion regions （SDRs） were detected among 8 different chromosomes, the relationship between X2 valueand position in chromosome of SNP was analyzed.

关 键 词：玉米 SNP 偏分离 加倍单倍体 

分 类 号：S513.035.2[农业科学—作物学]