玉米S型细胞质雄性不育系可溶性糖、游离脯氨酸及丙二醛含量分析  

Analysis of Soluble Sugar, Free Proline and MDA Content of S-cytoplasmic Male Sterile Line in Maize

作　　者：焦东宝 赵永锋[1] 郭晋杰[1] 寇凤仙[2] 贾晓艳[1] 黄亚群[1] 祝丽英[1] 陈景堂[1] 

机构地区：[1]河北农业大学农学院/国家玉米改良中心河北分中心/河北省作物种质资源实验室,河北保定071001 [2]保定职业技术学院,河北保定071051

出　　处：《玉米科学》2015年第3期66-70,75,共6页Journal of Maize Sciences

基　　金：国家"863"重点课题(2011AA10A103-3);河北省现代农业技术体系玉米创新团队项目

摘　　要：以玉米自交系郑58及其两个S型细胞质雄性不育系为材料,对不同生育时期叶片和雄穗小花中的可溶性糖、游离脯氨酸和丙二醛含量进行比较研究。结果表明,在散粉期,郑58叶片中可溶性糖含量显著低于不育系,雄穗小花中可溶性糖含量显著高于不育系。郑58叶片中游离脯氨酸含量仅在雄穗发育末期显著低于郑58cmsQ1261;雄穗小花中游离脯氨酸含量在雄穗发育初期及散粉期均显著高于不育系。郑58叶片中丙二醛含量除雄穗发育末期和抽雄期,其他时期与不育系均有显著差异;雄穗小花中丙二醛含量在雄穗发育初期和散粉期与不育系差异均不显著。Zheng58cms-M084 and Zheng58cms-Q1261 which were S-cytoplasmic male sterility lines and their maintainer Zheng58 were used to comparatively analyze the contents of soluble sugar, free proline and malondi- aldehyde（MDA） in their leaves and anthers during different developmental stages. The results showed that soluble sugar content in the leaves of Zheng58 was significantly lower than that of two sterile lines at pollen shedding stage, while the content of soluble sugar in the anthers of Zheng58 was significantly higher than that of two sterile lines. Free proline content in the leaves of Zheng58 was significantly lower than that of Zheng58cms-Q1261 only at the later stage of tassel florets development, while the content of free proline in the anthers of Zheng58 was significantly higher than that of two sterile lines at the early stage of tassel florets development and pollen shedding stage. MDA content in the leaves of Zheng58 was significant difference with two sterile lines during different developmental stag- es except the later stage of tassel florets development and tasselling stage, while the content of MDA in the an- thers of Zheng58 was not significant difference with two sterile lines at the early stage of tassel florets development and pollen shedding stage.

关 键 词：玉米 S型细胞质雄性不育 可溶性糖 游离脯氨酸 丙二醛 

分 类 号：S513.01[农业科学—作物学]