双水相分离纯化R-藻红蛋白  

Aqueous two-phase processes for separation and purification of R-phycoerythrin

作　　者：赵丽[1,2] 彭一良[2] 付秋爽 蔡伟民[1] 

机构地区：[1]哈尔滨工业大学市政环境工程学院,哈尔滨150090 [2]哈尔滨师范大学生命科学与技术学院,哈尔滨150025

出　　处：《哈尔滨工业大学学报》2015年第6期54-58,共5页Journal of Harbin Institute of Technology

摘　　要：为了获得优于食品级的R-藻红蛋白(R-PE),采用双水相体系从坛紫菜中分离纯化R-PE.结果表明,聚乙二醇(PEG)/酒石酸钾钠双水相体系,当PEG相对分子质量为1 500且p H为8.06、系线长22.30%、体积比0.12时纯化效果最佳,R-PE纯度由细胞破碎液的0.43提高到1.47,回收率为84.42%.经过二次双水相纯度达1.55,纯化因子为3.60.得到的R-PE具有较高的生物活性.PEG相对分子质量1500/酒石酸钾钠双水相体系成功分离纯化R-PE,操作简单,成本低,适于工业化.The phycoerythrin （R-PE） is a fluorescent protein, which has great commercial and industrial value. In order to obtain a high purity R-PE which above the food grade standard, the R-PE was separated and purified from the Porphyra haitanensis using aqueous two-phase system （ATPS）. At the optimal conditions of polyethylene glycol （PEG） of 1500 in the ATPS （potassium sodium tartrate/PEG）, pH of 8.06, the tie- line length （LTL） of 22.30% and volume ratio （V,） of 0.12, purity of the isolated R-PE would increase from 0.43 （initial purity） to 1.47 after the first extraction, with a recovery rate of 84.42%. After the second ATPS extraction, achieved a purity and purification factor of 1.55 and 3.60, respectively. The isolated R-PE had a high biological activity. It was a successful purification process for R-PE for industrial application, with the advantages of high efficient and low cost.

关 键 词：R-藻红蛋白 双水相 纯度 酒石酸钾钠 

分 类 号：Q51[生物学—生物化学] Q81