采用UPLC-TQ-MS同时测定银杏花粉24种资源性化学成分  

Determine resource chemical component in ginkgo pollen simultaneously by UPLC-TQ-MS

作　　者：徐澄梅 任浩[2] 钱大玮[2] 孙光天 宿树兰[2] 郭盛[2] 欧阳臻[1] 段金廒[2] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]南京中医药大学江苏省中药资源产业化过程协同创新中心/中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210023 [3]邳州市银杏科学研究所,江苏邳州221300

出　　处：《中国中药杂志》2015年第11期2157-2162,共6页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2011BAI04B03);国家教育部"新世纪优秀人才支持计划"项目(NCET-09-0163);江苏高校优势学科建设工程项目(ysxk-2010)

摘　　要：分析评价银杏花粉中黄酮类、萜内酯类、银杏酸类以及原花青素类化学成分。采用UPLC-TQ-MS的方法对银杏花粉中24种资源性化学成分进行定性定量分析。结果显示芦丁和槲皮素-3-O-β-D-吡喃葡萄糖-(4→1)-β-L-吡喃鼠李糖苷达到120.9μg·g-1和114.0μg·g-1,山柰酚为222.1μg·g-1。该文采用UPLC-TQ-MS的方法首次同时测定银杏花粉中24种资源性化学成分,此方法快速、便利,质量准确,为银杏花粉的资源化利用提供了基础性工作。The present study is to determine the flavonoid glycosides, terpene lactones, biflavones, gingko acid and procyanidins of ginkgo pollen. UPLC-TQ-MS technology was used for the determination of 24 kinds of resource chemical composition in ginkgo pollen qualitatively and quantitatively. The results shows that the contents of rutin, quercetion 3-O-[4-O-（α-L-rhamnosy1）-β-D-glucoside] and kaempferolis were 120.9, 114.0, 222.1 μg·g^-1. In this paper, the contents of 24 kinds of chemical components of ginkgo pollen were determinated by UPLC-TQ-MS for the first time. This method is simple and quick, which will be benefit for recycling utilization of ginkgo pollen.

关 键 词：银杏 花粉 黄酮类 萜内酯类 银杏酸类 资源化利用 

分 类 号：R284.1[医药卫生—中药学]