3种来源藏紫菀药材的形态学与薄层色谱鉴别研究  

Morphological and TLC identification on Tibetan medicine Asteris Flos

作　　者：丁锤[1] 张炜[2] 边樱 张朝凤[1] 许翔鸿[1] 张勉[1] 

机构地区：[1]中国药科大学生药学研究室,江苏南京211198 [2]青海省药品检验所,青海西宁810016

出　　处：《中国中药杂志》2015年第11期2244-2248,共5页China Journal of Chinese Materia Medica

基　　金：<中国药典>2015年版藏药药材质量标准修订项目

摘　　要：对来源于缘毛紫菀Aster souliei、萎软紫菀A.flaccidus和块根紫菀A.asteroides的藏紫菀药材进行了鉴别研究。参照《中国药典》2010年版附录相关方法,对药材进行了性状观察和描述,拍摄了药材照片;对药材粉末进行了显微观察,描述了主要显微特征,并制备了粉末显微图谱;以灯盏甲素和绿原酸为指标性成分,采用薄层色谱法建立了藏紫菀的薄层色谱鉴别方法。3种来源藏紫菀在药材性状和粉末显微特征方面均具有一定的区别,二者结合可以较好的区分3种不同的来源;薄层色谱鉴别结果显示,来源于缘毛紫菀和萎软紫菀的药材薄层色谱图较为一致,块根紫菀具有一定差别。该实验建立的性状、显微和薄层色谱鉴别方法简便、准确、重复性好,可方便、有效地区别3种不同来源的藏紫菀药材。Tibetan medicine Asteris Flos is the flowers of Aster souliei, A. flaccidus, and A. asteroides, with the function of clearing away heat and toxic matter, relieving cough, and removing phlegm. In order to control the quality of Asteris Flos, the morphological and chemical methods were established for identification of three origins. The morphological features of three species were described and photographed, and the microscopic characteristics of three drug powders were also described in detail and pictured. The results showed that three origins of Asteris Flos could be easily distinguished by their macro- and micro-morphologic features, and a key for distinguishing the three origins was given. Moreover, a TLC method, with apigenin-7-O-β-D-glucurono pyranoside and chlorogenic acid as chemical references, was also established for the identification of three origins. The results showed that the TLC chromatograms of the flowers of A. souliei and A. flaccidus were very similar, but different from that of A. asteroides. The established macroscopic, powder microscopic and TLC methods for identification of three origins of AF were simple, accurate, and reproducible, and also effective and easy to operate.

关 键 词：藏紫菀 性状鉴定 显微鉴定 薄层色谱鉴别 灯盏甲素 

分 类 号：R29[医药卫生—民族医学] O657.7[医药卫生—中医学]