添加N-乙酰半胱氨酸对猪精子常温保存的影响  

Effect of the addition of N-acetyl cysteine on the preservation of pig sperm at room temperature

作　　者：张春龙[1] 周佳勃[1] 岳顺利[1] 张利[1] 于思淼 张朔[1] 张宇霆[1] 谭景和[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《黑龙江畜牧兽医》2015年第6期85-87,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金面上项目(31372311)

摘　　要：为了研究N-乙酰半胱氨酸(NAC)对猪精子液态常温保存的影响,试验向保存液中添加不同浓度的NAC(0.5 mmol/L、1 mmol/L、2 mmol/L、5 mmol/L),在15℃条件下保存猪精子,检测保存后的精子活率、活力和弯尾率。结果表明:在稀释液中添加1 mmol/L的NAC能显著提高精子的活率、活力、弯尾率(P<0.05)。通过添加NAC可以显著提高精子的保存能力。To investigate the effect of N - acetyl cysteine （NAC） on liquid preservation of pig sperm at room temperature, the different concentrations of DAC including 0.5 mmol/L, 1 mmol/L, 2 mmol/L and 5 mmol/L, were added into the preserving liquid to preserve pig sperm under the condition of 15℃. And then the sperm motility rate, viability and tail coiling rate were detected after preservation. The results showed that the sperm motility rate, viability and tail coiling rate were significantly increased （ P 〈 0.05 ） when adding 1 mmol/L N - acetyl cysteine in the preserving liquid. The results indicate that the addition of NAC can significantly improve the sperm preservation ability.

关 键 词：猪 精子 液态保存 N-乙酰半胱氨酸(NAC) 活性氧(ROS) 

分 类 号：S828[农业科学—畜牧学] S813[农业科学—畜牧兽医]