HPLC考察燕窝加工前后唾液酸的含量变化  

Study on the Content Changes of Sialic Acid in Bird 's Nest before and after Process by HPLC

作　　者：卓丹如 阙慧卿 林绥 章宁 钱丽萍 林善财 

机构地区：[1]福州瑞来春堂生物科技有限公司,福建福州350001 [2]福建省医学科学研究院

出　　处：《中国执业药师》2015年第6期17-20,共4页China Licensed Pharmacist

摘　　要：目的:建立燕窝中唾液酸的分离分析方法,考察燕窝原料(去除杂质的干燥燕窝)、炖煮和加工成即食罐头前后唾液酸的含量变化。方法:燕窝经过磷酸水解,使唾液酸(即N-乙酰神经氨酸)从唾液酸糖蛋白的结合状态游离,用高效液相色谱法测定唾液酸的含量,应用《中药色谱指纹图谱相似度评价系统》评价燕窝3种样品的指纹图谱,比较3种样品的唾液酸含量变化。结果:燕窝原料的唾液酸含量为9.898%,经炖煮或加工成即食罐头后,唾液酸的含量分别为9.921%,9.913%,略高于原料。结论:燕窝加工成即食罐头后其唾液酸含量与直接炖煮的接近,略高于燕窝原料,提示应用现代工艺将燕窝加工成即食罐头,唾液酸保存较好。Objective：To establish the separation and analysis methods of sialic acid in bird’s nest and investigate the content changes of sialic acid in raw material（dry bird’s nest after removal of impurities）and processed products of bird’s nest.Methods：Sialic acid（that is N-acetyl neuraminic acid）in bird’s nest was free from the combination of sialic acid glycoprotein through phosphoric acid hydrolysis. The content of sialic acid was determined by HPLC,and the evaluation was made on the fingerprints of three samples using theSimilarity EvaluationSystemforChromatographicFingerprintofTCM,and the content changes of sialic acid were compared. Results：Sialic acid content of bird’s nest raw material was 9.898% and which was 9.921% and 9.913% after process by stewing and making into canned bird’s nest,which was a little higher than the bird’s nest raw material.Conclusion：The content of sialic acid of processed bird’s nest was slightly higher than that of bird’s nest raw materials. It is indicated that sialic acid content can be better preserved in the ready-to-eat canned bird’s nest produced with modern technology.

关 键 词：燕窝原料 炖煮燕窝 燕窝即食罐头 唾液酸(N-乙酰神经氨酸) 高效液相色谱法 中药色谱指纹图谱相似度评价 SALIVA ACID (N-Acetyl Neuraminic Acid) 

分 类 号：R382.31[医药卫生—医学寄生虫学]