紫外光谱法测定白芍药材中白芍总苷的含量  

Determination of total glucoside in Paeonialactifloria Pall.by UV spectrophotometry

作　　者：刘芬[1] 侯聪聪[2] 

机构地区：[1]河北省邯郸市中心医院药学部,056001 [2]河北医科大学药学院,石家庄050017

出　　处：《国际中医中药杂志》2015年第6期532-534,共3页International Journal of Traditional Chinese Medicine

摘　　要：目的：建立紫外分光光度法测定白芍饮片中白芍总苷的含量。方法采用80%乙醇提取白芍饮片，提取液除去乙醇后，经乙醚萃取除去杂质，用正丁醇萃取制备供试品溶液。以芍药苷为对照品，在最大吸收波长230 nm处进行含量测定。结果芍药苷在0.0402～0.2011 mg/ml范围内线性关系良好。平均回收率为101.81%，精密度、稳定性、重复性、加样回收率试验中RSD值均小于2%。白芍中总苷含量约为40 mg/g。结论本方法简便、灵敏度高、准确性好，适用于白芍饮片中总苷的含量测定。ObjectiveA method was proposed for the determination of the concent of total glucoside of peaony（TGP） fromPaeonia lactifloriaPall. by UV spectrophotometry.MethodsBased on the extract with 80% acidities alcohol, extracted by aether and n-butyl alcohol, TGP was obtained fromPaeonialactifloriaPall.. The determination was established with paeoniflorin which was the main component of TGP as a contrast by UV spectrophotometry at 230 nm.ResultsA good liner relationship between concentrationof paeoniflorin and its absorbance was obtained,and the liner range was between 0.040 2~0.201 1 mg/ml. The averagerecovery was 101.81%.ConclusionThis method is simple rapid and reliable. It could be used for the semi-quantitative determination of TGP of paeony in paeonialactifloria pall..

关 键 词：白芍 紫外分光光度法 白芍总苷 含量测定 PAEONIA lactifloriapall. 

分 类 号：R284[医药卫生—中药学] O657.3[医药卫生—中医学]