红花的抗氧化活性成分研究  

Antioxidant Compounds from Safflower

作　　者：Bulgantuya Bumandorj Narantsetseg Byambaakhuu 叶日贵 岳丽丽[1] 何小丽[1] 马超美[1] 

机构地区：[1]内蒙古大学生命科学学院,呼和浩特010021

出　　处：《内蒙古大学学报（自然科学版）》2015年第3期301-307,共7页Journal of Inner Mongolia University：Natural Science Edition

基　　金：国家自然科学基金资助项目(81160511)

摘　　要：研究重要中蒙药材红花成分的分离、结构测定和抗氧化活性.从红花中分离鉴定出羟基红花黄色素A(1),6-羟基山奈酚-3,6-二葡萄糖苷(2),6-羟基山奈酚-3,6,7-三葡萄糖苷(3),6-羟基山奈酚-3-芦丁糖-6-葡萄糖苷(4),山奈酚-3-芦丁糖苷(5),山奈酚-3-葡萄糖苷(6),异槲皮素(7),绿原酸(8),紫丁香苷(9),原儿茶酸(10),对香豆酸(11)和8Z-decaene-4,6-diyne-1-O-β-D-glucopyranoside(12).通过LC-MS和NMR分析确定了这些化合物的结构.通过水解红花主要黄酮成分得到苷元:6-羟基山奈酚.抗氧化活性实验显示黄酮苷元对DPPH游离基的清除作用比其苷类显著增强.因为黄酮苷类可在体内转化为苷元,因此本研究结果提示6-羟基山奈酚是红花发挥体内疗效的重要物质基础.Safflower（petals of Carthamus tictorius）is an important traditional herbal drug.The present work focuses on the isolation,structure determination,and antioxidant activity of the chemical constituents of safflower.Hydroxysafflor yellow A,6-hydroxykaempferol-3,6,7-triglucoside,6-hydroxykaempferol-3-rutinoside-6-glucoside,6-hydroxykaempferol-3,6-diglucoside,kaempferol-3-Orutinoside,kaempferol-3-O-glucoside,isoquercitin,chlorogenic acid,syringin,protocatechuic acid,pcoumaric acid,and 8Z-decaene-4,6-diyne-1-O-β-D-glucopyranoside were isolated and identified.Their structures were determined by LC-MS and NMR analysis.The aglycone,6-hydroxykaempferol was obtained by hydrolysis of the major safflower flavonoid glycoside.Antioxidant test demonstrated that the flavonoid aglycones showed much stronger DPPH scavenging activity than the glycosides.The results suggest that 6-hydroxykaempferol and other flavonoid aglycones may play important roles for the in vivo bioactivity of safflower,as glycosides are known to metabolize to their aglycones in human.

关 键 词：红花 化学成分 黄酮类 6-羟基山奈酚 抗氧化 

分 类 号：R284[医药卫生—中药学]