1株基因3型鸭甲肝病毒鸡胚化弱毒株的培育  

Development of a Chicken-embryo-adapted Attenuated Duck Hepatitis A Virus 3

作　　者：谢小雨[1] 袁媛[1] 王府民[1] 张大丙[1] 

机构地区：[1]中国农业大学动物医学院农业部动物流行病学与人畜共患病重点实验室,北京海淀100193

出　　处：《中国兽医杂志》2015年第4期31-33,共3页Chinese Journal of Veterinary Medicine

基　　金：现代农业产业技术体系专项资金(CARS-43);公益性行业(农业)专项经费(201003012)

摘　　要：为研发基因3型鸭甲肝病毒(Duckhepatitis virustype3,DHAV-3)的弱毒活疫苗,用SPF鸡胚对DHAV-3Y株进行了连续传代致弱,由此获得第80代鸡胚适应毒。致病性试验结果显示,第80代毒株对1日龄雏鸭已无致病性。免疫攻毒保护试验显示,雏鸭在1日龄时经肌肉注射途径免疫第80代鸡胚适应毒,能有效抵抗7日龄时的强毒感染,保护指数为100%。表明第80代鸡胚适应毒是一株具有良好免疫原性的鸡胚化弱毒疫苗候选株。To develop a live attenuated vaccine against duck hepatitis A virus type 3 （DHAV-3） , a duck-embryo-originDHAV-3 isolate Y was attenuated by repeated passages in SPF chicken embryos for 80 times.The 80th passage virus was shown tobe non-pathogenic for one-day-old ducklings. Subsequently, it was used as a vaccine to inoculate one-day-old ducklings. Theducklings vaccinated with the 80th passage virus at one-day-old age via intramuscular injection were protected from challengewith virulent strain of DHAV-3 7 days after immunization, with protective index of 100%. It is concluded that the 80th passage vi-rus is an attenuated vaccine candidate of chicken-embryo-adapted DHAV-3 with a good immunogenicity.

关 键 词：鸭病毒性肝炎 鸭甲肝病毒 鸡胚化弱毒疫苗 

分 类 号：S852.65[农业科学—基础兽医学]