复脉膏外敷对大鼠长春瑞滨外渗性损伤组织中VEGF的影响  

Effects of Fumai Paste on VEGF of Tissue in Vinorelbine- induced Extravasation Injury Model Rats

作　　者：周留勇[1] 尤建良[1] 单珍珠[1] 张辰岑[1] 龚时夏[1] 

机构地区：[1]无锡市中医医院肿瘤科,无锡214071

出　　处：《世界中医药》2015年第4期549-552,556,共5页World Chinese Medicine

基　　金：无锡市科学技术局科技支撑-社会发展项目(编号:CSE01019)

摘　　要：目的:建立大鼠长春瑞滨外渗性组织损伤的理想实验模型,在此动物模型上研究复脉膏外敷对外渗性损伤组织中VEGF的影响,以探讨复脉膏外敷治疗化疗性静脉炎的疗效及机制。方法:42只大鼠随机分为2组:复脉膏组、50%硫酸镁组,21只/组,建立实验模型。2组大鼠左侧损伤处分别予复脉膏外敷、50%硫酸镁湿敷;右侧予生理盐水外敷作为对照组。在损伤后第1、4、7、11、14、18天,分别随机取3只大鼠,切除损伤部位的皮肤及皮下组织,固定标本,脱水、石蜡包埋,连续切片,采用免疫组化标记及图像分析方法,在电子显微镜下观察VEGF的表达情况。结果:VEGF阳性表达主要见于表皮细胞、血管内皮细胞、毛囊上皮细胞胞浆、渗出的巨噬细胞、增生的成纤维细胞胞浆。复脉膏外敷较硫酸镁湿敷及生理盐水外涂能明显刺激长春瑞滨外渗性损伤组织中VEGF的阳性表达(P<0.01)。结论:复脉膏外敷于损伤皮肤后能上调损伤组织中VEGF的表达,通过VEGF的生物学功能,进而促进损伤组织的愈合。因此,复脉膏可以用于防治化疗性静脉炎。Objective：To establish an ideal animal experiment model of vinorelbine-induced extravasation injury to study effects of Fumai Paste on VEGF of tissue,and explore the curative effect and mechanism of treating chemotherapeutic phlebitis with external application of Fumai Paste.Methods：Forty-two rats were randomly divided into two groups with 21 rats in each group.Each rat was established to experimental model.Rats in Fumai Paste group were treated with external application of Fumai Paste on left side;and rats in magnesium sulfate groiup were treated with hydropathic compress of 50% magnesium sulfate on left side.The right side of rats in both groups were treated with hydropathic compress of normal saline.Three rats were selected randomly from each group on day 1,day 4,day 7,day 11,day 14 and day 18,and the skin and subcutaneous tissue of extravasation injury of each rat was cut off.All specimens were fixed in formalin,dehydrated,embedded in paraffin and serially cut.All specimens were observed expres-sions of VEGF under the electron microscope by immunohistochemical assay and image analysis method.Results：The positive ex-pression of VEGF was major in epidermic cells,vascular endothelial cell,endochylema of hair follicle cells,macrophage,endochyle-ma of fibroblast.Fumai Paste could stimulate the positive expression of VEGF tissue of animal experiment model with vinorelbine-induced extravasation injury more than 50% magnesium sulfate and normal saline （P 〈0.01）.Conclusion：Fumai Paste can up-regulate the expression of VEGF tissue of animal experiment model with vinorelbine-induced extravasation injury.With the biologi-cal function of VEGF,Fumai Paste can accelerate the healing of the damaged tissues.Hence,chemotherapeutic phlebitis can be prevented and treated with external application of Fumai Paste.

关 键 词：复脉膏 长春瑞滨 外渗性损伤 VEGF 

分 类 号：R332[医药卫生—人体生理学] R273[医药卫生—基础医学]