人参总皂苷对野百合碱诱导的大鼠右室肥厚及ERK通路的影响  

Effects of Total Ginsenosides on Monocrotaline-induced Cardiac Hypertrophy and ERK Signaling Pathway in Rats

作　　者：秦娜[1] 魏立伟[1] 

机构地区：[1]河南省洛阳正骨医院,洛阳471000

出　　处：《世界中医药》2015年第4期560-563,共4页World Chinese Medicine

摘　　要：目的:观察人参总皂苷(Total Ginsenosides,TG)对野百合碱(Monocrotaline,MCT)所致大鼠右心室肥厚的影响并探讨其与细胞外信号调节激酶(ERK)信号通路的关系。方法:雄性SD大鼠随机分为正常对照组,MCT模型组,TG 20 mg/(kg·d)、40 mg/(kg·d)、60 mg/(kg·d)剂量组,各组动物均给药18 d。测定各组大鼠的右室肥厚指数(RVHI)、右心重/体重(RVW/BW);透射电镜观察心肌细胞超微结构的改变;Real time RT-PCR检测心肌组织ERK1mRNA的表达,Western blotting检测心肌组MKP-1蛋白质的表达。结果:TG预防给药均使RVHI、RV/BW表达明显降低,改善超微结构损伤,降低心肌组织ERK1mRNA和提高MKP-1蛋白质的表达。结论:TG能明显改善MCT诱导的大鼠右心室肥厚,其抗心肌肥厚作用至少与抑制ERK1信号转导通路有关。Objective：To investigate the inhibition of total ginsenosides （TG）on right ventricular hypertrophy induced by mono-crotaline（MCT）and explore the effects of TG on extracellular-regulated kinase（ERK）signal transduction in rat.Methods：Male Sprague Dawley rats were randomly divided into the normal group,MCT group and 20 mg/（kg·d）,40 mg/（kg·d）,60 mg/（kg ·d）dose TG treatment group.Ventricular hypertrophy index（RVHI）was measured by the weight ratio of RV to left ventricle plus septum,and by observing the ultrastructural changes of myocardial cell with transmission electron microscope.The ERK1 mRNA and mitogen-activated protein kinase phosphatase-1（MKP-1）protein expression of right ventricle tissue were detected by real-time RT-PCR,Western blotting.Results：TG treatment could significantly reduce RVHI,RVW/BW.The swollen and misshapen mito-chondria could be improved by TG.TG also could down-regulate ERK1 mRNA expression and enhance MKP-1 protein expression. Conclusion：TG could significantly attenuate MCT-induced cardiac hypertrophy in rat by suppressing ERK Signaling pathway.

关 键 词：人参总皂苷 右室肥厚 细胞外信号调节激酶 丝裂素活化蛋白激酶磷酸酶-1 

分 类 号：R285.5[医药卫生—中药学]