Nanostructured lipid carrier based topical gel of Ganoderma Triterpenoids for frostbite treatment  

作　　者：SHEN Cheng-Ying DAI Ling SHEN Bao-De ZHOU Xu BAI Jin-Xia XU He LV Qing-Yuan HAN Jin YUAN Hai-Long 

机构地区：[1]Department of Pharmacy, 302 Military Hospital [2]College of Pharmacy, Chengdu University of Traditional Chinese Medicine

出　　处：《Chinese Journal of Natural Medicines》2015年第6期454-460,共7页中国天然药物（英文版）

基　　金：supported by Beijing Natural Science Foundation of China(No.7122176);National Natural Science Foundation of China(No.81102821);National Key New Drugs Innovation Foundation(Nos.2014ZX09J14106-01A and CWS11J165)

摘　　要：The objective of this study was to prepare nanostructured lipid carrier(NLC)-based topical gel of Ganoderma Triterpenoids(GTs) and evaluate their effects on frostbite treatment. GT-NLCs was prepared by the high pressure homogenization method and then characterized by morphology and analyses of particle size, zeta potential, entrapment efficiency(EE), and drug loading(DL). The NLCs was suitably gelled for skin permeation studies in vitro and pharmacodynamic evaluation in vivo, compared with the GT emulgel. The GT-NLC remained within the colloidal range and was uniformly dispersed after suitably gelled by carbopol preparation. Transmission electron microscopy(TEM) study showed GT-NLCs was spherical in shape. The EE(%) and DL(%) could reach up to(81.84 ± 0.60)% and(2.13 ± 0.12)%, respectively. The result of X-ray diffractograms(XRD) showed that GTs were in an amorphous state in the NLC-gel. In vitro permeation studies through rat skin indicated that the amount of GTs permeated through skin of GT-NLCs after 24 h was higher than that of GT emulsion, and GT-NLCs increased the accumulative amounts of GTs in epidermis 7.76 times greater than GT emulsion. GT-NLC-gel was found to possess superior therapeutic effect for frostbite, compared with the GT emulgel. The NLC based topical gel of GTs could improve-their therapeutic effect for frostbite.The objective of this study was to prepare nanostmctured lipid carder （NLC）-based topical gel of Ganoderma Triterpenoids （GTs） and evaluate their effects on frostbite treatment. GT-NLCs was prepared by the high pressure homogenization method and then characterized by morphology and analyses of particle size, zeta potential, entrapment efficiency （EE）, and drug loading （DL）. The NLCs was suitably gelled for skin permeation studies in vitro and pharmacodynamic evaluation in vivo, compared with the GT emulgel. The GT-NLC remained within the colloidal range and was uniformly dispersed after suitably gelled by carbopol preparation. Transmission electron microscopy （TEM） study showed GT-NLCs was spherical in shape. The EE （%） and DL （%） could reach up to （81.84 ± 0.60）% and （2.13 ± 0.12）%, respectively. The result of X-ray diffractograms （XRD） showed that GTs were in an amorphous state in the NLC-gel. In vitro permeation studies through rat skin indicated that the amount of GTs permeated through skin of GT-NLCs after 24 h was higher than that of GT emulsion, and GT-NLCs increased the accumulative amounts of GTs in epidermis 7.76 times greater than GT emulsion. GT-NLC-gel was found to possess superior therapeutic effect for frostbite, compared with the GT emulgel. The NLC based topical gel of GTs could improve -their therapeutic effect for frostbite.

关 键 词：Nanostructured lipid carriers High pressure homogenization Topical gel Ganoderma Triterpenes FROSTBITE 

分 类 号：R283.6[医药卫生—中药学]